Anti-NGB Antibody (CAB6477)
- SKU:
- CAB6477
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Frequently bought together:
Description
| Antibody Name: | Anti-NGB Antibody |
| Antibody SKU: | CAB6477 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-151 of human NGB (NP_067080.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:10 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse liver, Mouse brain, Rat liver, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-151 of human NGB (NP_067080.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MERP EPEL IRQS WRAV SRSP LEHG TVLF ARLF ALEP DLLP LFQY NCRQ FSSP EDCL SSPE FLDH IRKV MLVI DAAV TNVE DLSS LEEY LASL GRKH RAVG VKLS SFST VGES LLYM LEKC LGPA FTPA TRAA WSQL YGAV VQAM SRGW DGE |
| Gene ID: | 58157 |
| Uniprot: | Q9NPG2 |
| Cellular Location: | Cytoplasm, Mitochondrion, Perikaryon |
| Calculated MW: | 16kDa |
| Observed MW: | 16kDa |
| Synonyms: | NGB |
| Background: | This gene encodes an oxygen-binding protein that is distantly related to members of the globin gene family. It is highly conserved among other vertebrates. It is expressed in the central and peripheral nervous system where it may be involved in increasing oxygen availability and providing protection under hypoxic/ischemic conditions. |
| UniProt Protein Function: | NGB: Involved in oxygen transport in the brain. Hexacoordinate globin, displaying competitive binding of oxygen or the distal His residue to the iron atom. Not capable of penetrating cell membranes. The deoxygenated form exhibits nitrite reductase activity inhibiting cellular respiration via NO-binding to cytochrome c oxidase. Involved in neuroprotection during oxidative stress. May exert its anti-apoptotic activity by acting to reset the trigger level of mitochondrial cytochrome c release necessary to commit the cells to apoptosis. Belongs to the globin family. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 14q24.3 Cellular Component: mitochondrion; perikaryon Molecular Function:iron ion binding; heme binding; oxygen binding; oxygen transporter activity Biological Process: apoptosis; oxygen transport |
| NCBI Summary: | This gene encodes an oxygen-binding protein that is distantly related to members of the globin gene family. It is highly conserved among other vertebrates. It is expressed in the central and peripheral nervous system where it may be involved in increasing oxygen availability and providing protection under hypoxic/ischemic conditions. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9NPG2 |
| NCBI GenInfo Identifier: | 32171399 |
| NCBI Gene ID: | 58157 |
| NCBI Accession: | Q9NPG2.1 |
| UniProt Related Accession: | Q9NPG2 |
| Molecular Weight: | 151 |
| NCBI Full Name: | Neuroglobin |
| NCBI Synonym Full Names: | neuroglobin |
| NCBI Official Symbol: | NGB |
| NCBI Protein Information: | neuroglobin |
| UniProt Protein Name: | Neuroglobin |
| Protein Family: | Neuroglobin |
| UniProt Gene Name: | NGB |
| UniProt Entry Name: | NGB_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NGB antibody (CAB6477) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of U2OS cells using NGB antibody (CAB6477). Blue: DAPI for nuclear staining. |
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