Anti-NFYB Antibody (CAB7295)
- SKU:
- CAB7295
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| Antibody Name: | Anti-NFYB Antibody |
| Antibody SKU: | CAB7295 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-207 of human NFYB (NP_006157.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HL-60, SKOV3, HeLa, Mouse liver, Mouse spleen, Mouse brain, Mouse thymus |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-207 of human NFYB (NP_006157.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MTMD GDSS TTDA SQLG ISAD YIGG SHYV IQPH DDTE DSMN DHED TNGS KESF REQD IYLP IANV ARIM KNAI PQTG KIAK DAKE CVQE CVSE FISF ITSE ASER CHQE KRKT INGE DILF AMST LGFD SYVE PLKL YLQK FREA MKGE KGIG GAVT ATDG LSEE LTEE AFTN QLPA GLIT TDGQ QQNV MVYT TSYQ QISG VQQI QFS |
| Gene ID: | 4801 |
| Uniprot: | P25208 |
| Cellular Location: | Nucleus |
| Calculated MW: | 22kDa |
| Observed MW: | 28-30kDa |
| Synonyms: | NFYB, CBF-A, CBF-B, HAP3, NF-YB |
| Background: | The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. |
| UniProt Protein Function: | NFYB: Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes. Belongs to the NFYB/HAP3 subunit family. |
| UniProt Protein Details: | Protein type:Transcription factor; DNA-binding Chromosomal Location of Human Ortholog: 12q22-q23 Cellular Component: nucleoplasm; CCAAT-binding factor complex; nucleus Molecular Function:protein binding; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; protein complex binding; transcription factor activity Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; cellular lipid metabolic process |
| NCBI Summary: | The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P25208 |
| NCBI GenInfo Identifier: | 399193 |
| NCBI Gene ID: | 4801 |
| NCBI Accession: | P25208.2 |
| UniProt Secondary Accession: | P25208,Q96IY8, A8K7B9, |
| UniProt Related Accession: | P25208 |
| Molecular Weight: | 207 |
| NCBI Full Name: | Nuclear transcription factor Y subunit beta |
| NCBI Synonym Full Names: | nuclear transcription factor Y, beta |
| NCBI Official Symbol: | NFYB |
| NCBI Official Synonym Symbols: | HAP3; CBF-A; CBF-B; NF-YB |
| NCBI Protein Information: | nuclear transcription factor Y subunit beta; Transcription factor NF-Y, B subunit; CAAT box DNA-binding protein subunit B; nuclear transcription factor Y subunit B; CCAAT-binding transcription factor subunit A |
| UniProt Protein Name: | Nuclear transcription factor Y subunit beta |
| UniProt Synonym Protein Names: | CAAT box DNA-binding protein subunit B; Nuclear transcription factor Y subunit B; NF-YB |
| Protein Family: | Nuclear transcription factor |
| UniProt Gene Name: | NFYB |
| UniProt Entry Name: | NFYB_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NFYB antibody (CAB7295) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of A-549 cells using NFYB antibody (CAB7295). Blue: DAPI for nuclear staining. |
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