Anti-NFS1 Antibody (CAB6668)
- SKU:
- CAB6668
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-NFS1 Antibody |
| Antibody SKU: | CAB6668 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 208-457 of human NFS1 (NP_066923.3). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SH-SY5Y, HepG2, BxPC-3, Mouse brain, Mouse liver, Mouse heart, Rat brain, Rat liver, Rat heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 208-457 of human NFS1 (NP_066923.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | EIGV KQPI AEIG RICS SRKV YFHT DAAQ AVGK IPLD VNDM KIDL MSIS GHKI YGPK GVGA IYIR RRPR VRVE ALQS GGGQ ERGM RSGT VPTP LVVG LGAA CEVA QQEM EYDH KRIS KLSE RLIQ NIMK SLPD VVMN GDPK HHYP GCIN LSFA YVEG ESLL MALK DVAL SSGS ACTS ASLE PSYV LRAI GTDE DLAH SSIR FGIG RFTT EEEV DYTV EKCI QHVK RLRE MSPL WEMV QDGI DLKS IKWT QH |
| Gene ID: | 9054 |
| Uniprot: | Q9Y697 |
| Cellular Location: | Cytoplasm, Mitochondrion, Nucleus |
| Calculated MW: | 44kDa/50kDa |
| Observed MW: | 47KDa |
| Synonyms: | NFS1, HUSSY-08, IscS, NIFS |
| Background: | Iron-sulfur clusters are required for the function of many cellular enzymes. The proteins encoded by this gene supply inorganic sulfur to these clusters by removing the sulfur from cysteine, creating alanine in the process. This gene uses alternate in-frame translation initiation sites to generate mitochondrial forms and cytoplasmic/nuclear forms. Selection of the alternative initiation sites is determined by the cytosolic pH. The encoded proteins belong to the class-V family of pyridoxal phosphate-dependent aminotransferases. Alternatively spliced transcript variants have been described. |
| UniProt Protein Function: | NFS1: Catalyzes the removal of elemental sulfur from cysteine to produce alanine. It supplies the inorganic sulfur for iron- sulfur (Fe-S) clusters. May be involved in the biosynthesis of molybdenum cofactor. Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily. 2 isoforms of the human protein are produced by alternative initiation. |
| UniProt Protein Details: | Protein type:EC 2.8.1.7; Cofactor and Vitamin Metabolism - thiamine; Transferase; Mitochondrial Chromosomal Location of Human Ortholog: 20q11.22 Cellular Component: nucleoplasm; intracellular membrane-bound organelle; mitochondrion; mitochondrial matrix; cytoplasm; nucleolus; cytosol; nucleus Molecular Function:protein binding; protein homodimerization activity; cysteine desulfurase activity; pyridoxal phosphate binding Biological Process: iron incorporation into metallo-sulfur cluster; cysteine metabolic process; vitamin metabolic process; sulfur amino acid metabolic process; protein complex assembly; Mo-molybdopterin cofactor biosynthetic process; molybdopterin cofactor biosynthetic process; water-soluble vitamin metabolic process |
| NCBI Summary: | Iron-sulfur clusters are required for the function of many cellular enzymes. The proteins encoded by this gene supply inorganic sulfur to these clusters by removing the sulfur from cysteine, creating alanine in the process. This gene uses alternate in-frame translation initiation sites to generate mitochondrial forms and cytoplasmic/nuclear forms. Selection of the alternative initiation sites is determined by the cytosolic pH. The encoded proteins belong to the class-V family of pyridoxal phosphate-dependent aminotransferases. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2010] |
| UniProt Code: | Q9Y697 |
| NCBI GenInfo Identifier: | 62512153 |
| NCBI Gene ID: | 9054 |
| NCBI Accession: | Q9Y697.3 |
| UniProt Secondary Accession: | Q9Y697,Q6P0L8, Q9NTZ5, Q9Y481, B3KMA5, B4DXK9, E1P5R8 F5GYK5, |
| UniProt Related Accession: | Q9Y697 |
| Molecular Weight: | 457 |
| NCBI Full Name: | Cysteine desulfurase, mitochondrial |
| NCBI Synonym Full Names: | NFS1 cysteine desulfurase |
| NCBI Official Symbol: | NFS1 |
| NCBI Official Synonym Symbols: | IscS; NIFS; HUSSY-08 |
| NCBI Protein Information: | cysteine desulfurase, mitochondrial; NFS1 nitrogen fixation 1 homolog; nitrogen-fixing bacteria S-like protein; nitrogen fixation 1 (S. cerevisiae, homolog) |
| UniProt Protein Name: | Cysteine desulfurase, mitochondrial |
| Protein Family: | Cysteine desulfurase |
| UniProt Gene Name: | NFS1 |
| UniProt Entry Name: | NFS1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NFS1 Polyclonal Antibody (CAB6668) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s. |
 | Immunohistochemistry of paraffin-embedded rat brain using NFS1 Rabbit pAb (CAB6668) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of C6 cells using NFS1 Rabbit pAb (CAB6668) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L929 cells using NFS1 Rabbit pAb (CAB6668) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-251 MG cells using NFS1 Rabbit pAb (CAB6668) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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