Anti-NFASC Antibody (CAB3053)
- SKU:
- CAB3053
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-NFASC Antibody |
| Antibody SKU: | CAB3053 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 830-1100 of human NFASC (NP_001005388.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SH-SY5Y, HeLa, HL-60, Mouse brain, Mouse pancreas, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 830-1100 of human NFASC (NP_001005388.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | RFRV RQPN LETI NLEW DHPE HPNG IMIG YTLK YVAF NGTK VGKQ IVEN FSPN QTKF TVQR TDPV SRYR FTLS ARTQ VGSG EAVT EESP APPN EATP TAAP PTLP PTTV GATG AVSS TDAT AIAA TTEA TTVP IIPT VAPT TIAT TTTV ATTT TTTA AATT TTES PPTT TSGT KIHE SAPD EQSI WNVT VLPN SKWA NITW KHNF GPGT DFVV EYID SNHT KKTV PVKA QAQP IQLT DLYP GMTY TLRV YSRD NEGI SSTV ITFM TSTA YTN |
| Gene ID: | 23114 |
| Uniprot: | O94856 |
| Cellular Location: | Cell membrane, Single-pass type I membrane protein |
| Calculated MW: | 69kDa/119kDa/130-150kDa |
| Observed MW: | 150kDa |
| Synonyms: | NFASC, NF, NRCAML |
| Background: | This gene encodes an L1 family immunoglobulin cell adhesion molecule with multiple IGcam and fibronectin domains. The protein functions in neurite outgrowth, neurite fasciculation, and organization of the axon initial segment (AIS) and nodes of Ranvier on axons during early development. Both the AIS and nodes of Ranvier contain high densities of voltage-gated Na+ (Nav) channels which are clustered by interactions with cytoskeletal and scaffolding proteins including this protein, gliomedin, ankyrin 3 (ankyrin-G), and betaIV spectrin. This protein links the AIS extracellular matrix to the intracellular cytoskeleton. This gene undergoes extensive alternative splicing, and the full-length nature of some variants has not been determined. |
| UniProt Protein Function: | neurofascin: Cell adhesion, ankyrin-binding protein which may be involved in neurite extension, axonal guidance, synaptogenesis, myelination and neuron-glial cell interactions. Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family. 13 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 1q32.1 Cellular Component: focal adhesion; paranode region of axon; integral to membrane; plasma membrane; intracellular Molecular Function:protein binding Biological Process: myelination; heterotypic cell-cell adhesion; axon guidance; clustering of voltage-gated sodium channels; synapse organization and biogenesis; transmission of nerve impulse; peripheral nervous system development; paranodal junction assembly |
| NCBI Summary: | This gene encodes an L1 family immunoglobulin cell adhesion molecule with multiple IGcam and fibronectin domains. The protein functions in neurite outgrowth, neurite fasciculation, and organization of the axon initial segment (AIS) and nodes of Ranvier on axons during early development. Both the AIS and nodes of Ranvier contain high densities of voltage-gated Na+ (Nav) channels which are clustered by interactions with cytoskeletal and scaffolding proteins including this protein, gliomedin, ankyrin 3 (ankyrin-G), and betaIV spectrin. This protein links the AIS extracellular matrix to the intracellular cytoskeleton. This gene undergoes extensive alternative splicing, and the full-length nature of some variants has not been determined.[provided by RefSeq, May 2009] |
| UniProt Code: | O94856 |
| NCBI GenInfo Identifier: | 83304988 |
| NCBI Gene ID: | 23114 |
| NCBI Accession: | O94856.4 |
| UniProt Secondary Accession: | O94856,Q149P5, Q5T2F0, Q5T2F1, Q5T2F2, Q5T2F3, Q5T2F4 B2RNN8, B3KQZ1, B5MDP6, B5MDR6, B7ZMD8, |
| UniProt Related Accession: | O94856 |
| Molecular Weight: | 1240 |
| NCBI Full Name: | Neurofascin |
| NCBI Synonym Full Names: | neurofascin |
| NCBI Official Symbol: | NFASC |
| NCBI Official Synonym Symbols: | NF; NRCAML |
| NCBI Protein Information: | neurofascin; neurofascin homolog |
| UniProt Protein Name: | Neurofascin |
| Protein Family: | Neurofascin |
| UniProt Gene Name: | NFASC |
| UniProt Entry Name: | NFASC_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NFASC antibody (CAB3053) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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