Anti-NF-kB2 Antibody (CAB3108)
- SKU:
- CAB3108
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-NF-kB2 Antibody |
| Antibody SKU: | CAB3108 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 690-899 of human NF-?B2 (NP_002493.3). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, Jurkat, H460, 293T, Mouse thymus |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 690-899 of human NF-?B2 (NP_002493.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | AGAD IHAE NEEP LCPL PSPP TSDS DSDS EGPE KDTR SSFR GHTP LDLT CSTK VKTL LLNA AQNT MEPP LTPP SPAG PGLS LGDT ALQN LEQL LDGP EAQG SWAE LAER LGLR SLVD TYRQ TTSP SGSL LRSY ELAG GDLA GLLE ALSD MGLE EGVR LLRG PETR DKLP STEV KEDS AYGS QSVE QEAE KLGP PPEP PGGL CHGH PQPQ VH |
| Gene ID: | 4791 |
| Uniprot: | Q00653 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 47kDa/96kDa |
| Observed MW: | 110kDa |
| Synonyms: | CVID10, H2TF1, LYT-10, LYT10, NF-kB2, p100, p49/p100, p52 |
| Background: | This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner. The p100 full-length protein is co-translationally processed into a p52 active form. Chromosomal rearrangements and translocations of this locus have been observed in B cell lymphomas, some of which may result in the formation of fusion proteins. There is a pseudogene for this gene on chromosome 18. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | NFkB-p100: a transcription factor of the nuclear factor-kappaB ( NFkB) group. Precursor of the p52 subunit of the nuclear factor NF-kappa-B, which binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. The precursor protein itself does not bind to DNA. LT-beta receptor agonists and LPS induce NF-kappaB/p100 processing to p52 at the level of the ribosome. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Two spliced isoforms have been identified. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. |
| UniProt Protein Details: | Protein type:Transcription factor; Oncoprotein; DNA-binding Chromosomal Location of Human Ortholog: 10q24 Cellular Component: Bcl3/NF-kappaB2 complex; nucleoplasm; cytoplasm; nucleus; cytosol Molecular Function:protein binding; transcription coactivator activity; chromatin binding; transcription factor activity Biological Process: spleen development; I-kappaB kinase/NF-kappaB cascade; extracellular matrix organization and biogenesis; transcription, DNA-dependent; follicular dendritic cell differentiation; MyD88-independent toll-like receptor signaling pathway; rhythmic process; toll-like receptor 3 signaling pathway; negative regulation of transcription from RNA polymerase II promoter; toll-like receptor 10 signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; regulation of transcription, DNA-dependent; response to cytokine stimulus; toll-like receptor signaling pathway; germinal center formation; positive regulation of interferon type I production; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; inflammatory response; toll-like receptor 4 signaling pathway Disease: Immunodeficiency, Common Variable, 10 |
| NCBI Summary: | This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner. The p100 full-length protein is co-translationally processed into a p52 active form. Chromosomal rearrangements and translocations of this locus have been observed in B cell lymphomas, some of which may result in the formation of fusion proteins. There is a pseudogene for this gene on chromosome 18. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013] |
| UniProt Code: | Q00653 |
| NCBI GenInfo Identifier: | 116242678 |
| NCBI Gene ID: | 4791 |
| NCBI Accession: | Q00653.4 |
| UniProt Secondary Accession: | Q00653,Q04860, Q9BU75, Q9H471, Q9H472, A8K9D9, D3DR83 |
| UniProt Related Accession: | Q00653,AAB23437 |
| Molecular Weight: | 900 |
| NCBI Full Name: | Nuclear factor NF-kappa-B p100 subunit |
| NCBI Synonym Full Names: | nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100) |
| NCBI Official Symbol: | NFKB2 |
| NCBI Official Synonym Symbols: | p52; p100; H2TF1; LYT10; CVID10; LYT-10; NF-kB2 |
| NCBI Protein Information: | nuclear factor NF-kappa-B p100 subunit; oncogene Lyt-10; NFKB, p52/p100 subunit; DNA-binding factor KBF2; transcription factor NFKB2; nuclear factor Kappa-B, subunit 2; nuclear factor NF-kappa-B p52 subunit; lymphocyte translocation chromosome 10 protein; nuclear factor of Kappa light chain gene enhancer in B cells 2 |
| UniProt Protein Name: | Nuclear factor NF-kappa-B p100 subunit |
| UniProt Synonym Protein Names: | DNA-binding factor KBF2; H2TF1; Lymphocyte translocation chromosome 10 protein; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2; Oncogene Lyt-10; Lyt10 |
| Protein Family: | Nuclear factor |
| UniProt Gene Name: | NFKB2 |
| UniProt Entry Name: | NFKB2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NF-kB2 antibody (CAB3108) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded rat brain using NF-kB2 antibody (CAB3108) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human breast using NF-kB2 antibody (CAB3108) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse testis using NF-kB2 antibody (CAB3108) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using NF-kB2 antibody (CAB3108) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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