Anti-NF-kB p65 Antibody (CAB2547)

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SKU:
CAB2547
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology
Frequently bought together:

Description

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Antibody Name:Anti-NF-kB p65 Antibody
Antibody SKU:CAB2547
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 50-180 of human NF-kB p65 (NP_068810.3).
Application:WB IHC IF IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, 293T, MCF7, Mouse lung, Mouse kidney, Rat lung, Rat kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 50-180 of human NF-kB p65 (NP_068810.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:RSTD TTKT HPTI KING YTGP GTVR ISLV TKDP PHRP HPHE LVGK DCRD GFYE AELC PDRC IHSF QNLG IQCV KKRD LEQA ISQR IQTN NNPF QVPI EEQR GDYD LNAV RLCF QVTV RDPS GRPL RLPP VLS
Gene ID:5970
Uniprot:Q04206
Cellular Location:Cytoplasm, Nucleus
Calculated MW:58kDa/59kDa/60kDa
Observed MW:65KDa
Synonyms:NFKB3, p65, NF-kB p65, RELA, CMCU
Background:NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:NFkB-p65: a subunit of NF-kappa-B transcription complex, which plays a crucial role in inflammatory and immune responses. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. P65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Three splice-variant isoforms have been identified.
UniProt Protein Details:

Protein type:DNA-binding; Nuclear receptor co-regulator; Transcription factor

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: cytoplasm; cytosol; nuclear chromatin; nucleoplasm; nucleus; transcription factor complex

Molecular Function:actinin binding; chromatin binding; chromatin DNA binding; DNA binding; histone deacetylase binding; identical protein binding; NF-kappaB binding; phosphate binding; protein binding; protein heterodimerization activity; protein homodimerization activity; protein kinase binding; protein N-terminus binding; RNA polymerase II transcription factor activity, enhancer binding; transcription activator binding; transcription factor activity; transcription factor binding; ubiquitin protein ligase binding

Biological Process: activation of NF-kappaB transcription factor; cytokine and chemokine mediated signaling pathway; inflammatory response; membrane protein intracellular domain proteolysis; negative regulation of apoptosis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of cell proliferation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interferon type I production; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of inflammatory response; response to organic substance; response to UV-B; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway

NCBI Summary:NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
UniProt Code:Q04206
NCBI GenInfo Identifier:62906901
NCBI Gene ID:5970
NCBI Accession:Q04206.2
UniProt Secondary Accession:Q04206,Q6GTV1, Q6SLK1,
UniProt Related Accession:Q04206
Molecular Weight:59,910 Da
NCBI Full Name:Transcription factor p65
NCBI Synonym Full Names:RELA proto-oncogene, NF-kB subunit
NCBI Official Symbol:RELA  
NCBI Official Synonym Symbols:p65; NFKB3  
NCBI Protein Information:transcription factor p65
UniProt Protein Name:Transcription factor p65
UniProt Synonym Protein Names:Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
Protein Family:Proline-rich P65 protein
UniProt Gene Name:RELA  
Anti-NF-kB p65 Antibody (CAB2547)Western blot analysis of extracts of various cell lines, using NF-kB p65 Rabbit pAb (CAB2547) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
Anti-NF-kB p65 Antibody (CAB2547)Immunohistochemistry of paraffin-embedded human stomach using NF-kB p65 Antibody (CAB2547) at dilution of 1:200 (40x lens).
Anti-NF-kB p65 Antibody (CAB2547)Immunofluorescence analysis of C6 cells using NF-kB p65 antibody (CAB2547) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-NF-kB p65 Antibody (CAB2547)Immunofluorescence analysis of L929 cells using NF-kB p65 antibody (CAB2547) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-NF-kB p65 Antibody (CAB2547)Immunofluorescence analysis of U2OS cells using NF-kB p65 antibody (CAB2547) at dilution of 1:100. Blue: DAPI for nuclear staining.

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