Anti-NET1 항체 (CAB1213)

Antibody Name:	Anti-NET1 Antibody
Antibody SKU:	CAB1213
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human NET1 (NP_001040625.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, SW480, MCF7, Mouse thymus, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human NET1 (NP_001040625.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MEPE LAAQ KQPR PRRR SRRA SGLS TEGA TGPS ADTS GSEL DGRC SLRR GSSF TFLT PGPN WDFT LKRK RREK DDDV VSLS SLDL KEPS NKRV RPLA RVTS LANL ISPV RNGA VRRF GQTI QSFT LRGD HRSP ASAQ KFSS RSTV PTPA KRRS SALW SEML DITM KESL TTRE IRRQ EAIY EMSR GEQD LIED LKLA RKAY HDPM LKLS IMSE EELT HIFG DLDS YIPL HEDL LTRI GEAT KPDG TVEQ IGHI LVSW LPRL NAYR GYCS NQLA AKAL LDQK KQDP RVQD FLQR CLES PFSR
Gene ID:	10276
Uniprot:	Q7Z628
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	61kDa/67kDa
Observed MW:	60kDa

Synonyms:

NET1, ARHGEF8, NET1A

Background:

This gene is part of the family of Rho guanine nucleotide exchange factors. Members of this family activate Rho proteins by catalyzing the exchange of GDP for GTP. The protein encoded by this gene interacts with RhoA within the cell nucleus and may play a role in repairing DNA damage after ionizing radiation. Pseudogenes of this gene are located on the long arms of chromosomes 1, 7 and 18. Alternative splicing results in multiple transcript variants that encode different protein isoforms.

UniProt Protein Function:	NET1: Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the SAPK/JNK pathway Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Oncoprotein; GEFs, Rac/Rho; GEFs; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 10p15 Cellular Component: plasma membrane; nucleus; cytosol Molecular Function:Rho guanyl-nucleotide exchange factor activity; guanyl-nucleotide exchange factor activity; GTP-Rho binding Biological Process: regulation of small GTPase mediated signal transduction; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; small GTPase mediated signal transduction; myoblast migration; regulation of cell growth; signal transduction
NCBI Summary:	This gene is part of the family of Rho guanine nucleotide exchange factors. Members of this family activate Rho proteins by catalyzing the exchange of GDP for GTP. The protein encoded by this gene interacts with RhoA within the cell nucleus and may play a role in repairing DNA damage after ionizing radiation. Pseudogenes of this gene are located on the long arms of chromosomes 1, 7 and 18. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jul 2012]
UniProt Code:	Q7Z628
NCBI GenInfo Identifier:	52782735
NCBI Gene ID:	10276
NCBI Accession:	Q7Z628.1
UniProt Secondary Accession:	Q7Z628,Q12773, Q96D82, Q99903, Q9UEN6,
UniProt Related Accession:	Q7Z628
Molecular Weight:	596
NCBI Full Name:	Neuroepithelial cell-transforming gene 1 protein
NCBI Synonym Full Names:	neuroepithelial cell transforming 1
NCBI Official Symbol:	NET1
NCBI Official Synonym Symbols:	NET1A; ARHGEF8
NCBI Protein Information:	neuroepithelial cell-transforming gene 1 protein; p65 Net1 proto-oncogene protein; small GTP-binding protein regulator; neuroepithelioma transforming gene 1; Rho guanine nucleotide exchange factor (GEF) 8; guanine nucleotide regulatory protein (oncogene)
UniProt Protein Name:	Neuroepithelial cell-transforming gene 1 protein
UniProt Synonym Protein Names:	Proto-oncogene p65 Net1; Rho guanine nucleotide exchange factor 8
Protein Family:	Nucleolar protein
UniProt Gene Name:	NET1
UniProt Entry Name:	ARHG8_HUMAN

	Western blot analysis of extracts of various cell lines, using NET1 antibody (CAB1213) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
	Immunofluorescence analysis of HeLa cells using NET1 antibody (CAB1213). Blue: DAPI for nuclear staining.

Antibodies

NET1 Antibody (PACO10821)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)