Anti-NEK4 Antibody (CAB16433)
- SKU:
- CAB16433
- Product type:
- Antibody
- Reactivity:
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| Antibody Name: | Anti-NEK4 Antibody |
| Antibody SKU: | CAB16433 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human NEK4 (NP_003148.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse testis, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human NEK4 (NP_003148.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MPLA AYCY LRVV GKGS YGEV TLVK HRRD GKQY VIKK LNLR NASS RERR AAEQ EAQL LSQL KHPN IVTY KESW EGGD GLLY IVMG FCEG GDLY RKLK EQKG QLLP ENQV VEWF VQIA MALQ YLHE KHIL HRDL KTQN VFLT RTNI IKVG DLGI ARVL ENHC DMAS TLIG TPYY MSPE LFSN KPYN YKSD VWAL GCCV YEMA TLKH AFNA KDMN SLVY RIIE GKLP PMPR DYSP ELAE LIRT MLSK RPEE RPSV RSIL RQPY IKRQ ISFF LEAT KIKT SKNN IKNG DSQS KPFA TVVS GEAE |
| Gene ID: | 6787 |
| Uniprot: | P51957 |
| Cellular Location: | Cell projection, Cytoplasm, cilium |
| Calculated MW: | 84kDa/88kDa/94kDa |
| Observed MW: | 95kDa |
| Synonyms: | NEK4, NRK2, STK2, pp12301 |
| Background: | The protein encoded by this gene is a serine/threonine protein kinase required for normal entry into replicative senescence. The encoded protein also is involved in cell cycle arrest in response to double-stranded DNA damage. Finally, this protein plays a role in maintaining cilium integrity, and defects in this gene have been associated with ciliopathies. |
| UniProt Protein Function: | NEK4: a protein kinase of the NEK family. Highly conserved between the mouse and human and expressed at high levels in the testis. |
| UniProt Protein Details: | Protein type:Protein kinase, Other; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Other group; NEK family Chromosomal Location of Human Ortholog: 3p21.1 Cellular Component: cytoplasm; cilium Molecular Function:protein serine/threonine kinase activity; manganese ion binding; ATP binding; receptor signaling protein serine/threonine kinase activity Biological Process: mitosis; cell division; mitotic cell cycle; protein amino acid phosphorylation; response to DNA damage stimulus |
| UniProt Code: | P51957 |
| NCBI GenInfo Identifier: | 116283241 |
| NCBI Gene ID: | 6787 |
| NCBI Accession: | |
| UniProt Related Accession: | P51957,Q05DF6 |
| Molecular Weight: | 50,822 Da |
| NCBI Full Name: | NEK4 protein |
| NCBI Synonym Full Names: | NIMA (never in mitosis gene a)-related kinase 4 |
| NCBI Official Symbol: | NEK4 |
| NCBI Official Synonym Symbols: | NRK2; STK2; pp12301; MGC33171 |
| NCBI Protein Information: | serine/threonine-protein kinase Nek4; OTTHUMP00000213580; OTTHUMP00000213581; OTTHUMP00000213582; serine/threonine kinase 2; nimA-related protein kinase 4; serine/threonine protein kinase-2; serine/threonine-protein kinase 2; never in mitosis A-related kinase 4; serine/threonine-protein kinase NRK2 |
| UniProt Protein Name: | NEK4 protein |
| Protein Family: | Serine/threonine-protein kinase |
| UniProt Gene Name: | NEK4 |
| UniProt Entry Name: | Q05DF6_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NEK4 antibody (CAB16433) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
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