Anti-NEFL Antibody (CAB0257)
- SKU:
- CAB0257
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-NEFL Antibody |
| Antibody SKU: | CAB0257 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 400-543 of human NEFL (NP_006149.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF7, 293T, Mouse brain, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 400-543 of human NEFL (NP_006149.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LSFT SVGS ITSG YSQS SQVF GRSA YGGL QTSS YLMS TRSF PSYY TSHV QEEQ IEVE ETIE AAKA EEAK DEPP SEGE AEEE EKDK EEAE EEEA AEEE EAAK EESE EAKE EEEG GEGE EGEE TKEA EEEE KKVE GAGE EQAA KKKD |
| Gene ID: | 4747 |
| Uniprot: | P07196 |
| Cellular Location: | |
| Calculated MW: | 61kDa |
| Observed MW: | 72kDa/68kDa |
| Synonyms: | NEFL, CMT1F, CMT2E, NF-L, NF68, NFL, PPP1R110 |
| Background: | Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. |
| UniProt Protein Function: | NFL: one of the three (L, M, and H) intermediate filament proteins that form neurofilaments. Neurofilaments are involved in the maintenance of neuronal caliber. NF-L is the most abundant of the three neurofilament proteins. Defects cause Charcot-Marie-Tooth disease type 1F (CMT1F). |
| UniProt Protein Details: | Protein type:Cytoskeletal Chromosomal Location of Human Ortholog: 8p21 Cellular Component: TSC1-TSC2 complex; growth cone; axon; cytoplasm; neurofilament; cytosol Molecular Function:protein C-terminus binding; protein binding, bridging; identical protein binding; protein domain specific binding; protein binding; phospholipase binding; structural constituent of cytoskeleton Biological Process: protein polymerization; response to peptide hormone stimulus; neurofilament bundle assembly; response to toxin; intermediate filament organization; microtubule cytoskeleton organization and biogenesis; retrograde axon cargo transport; axon regeneration in the peripheral nervous system; synaptic transmission; axon transport of mitochondrion; regulation of axon diameter; positive regulation of axonogenesis; negative regulation of neuron apoptosis; anterograde axon cargo transport; neuromuscular process controlling balance; locomotion; neurite morphogenesis; response to corticosterone stimulus Disease: Charcot-marie-tooth Disease, Axonal, Type 2e; Charcot-marie-tooth Disease, Demyelinating, Type 1f |
| NCBI Summary: | Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008] |
| UniProt Code: | P07196 |
| NCBI GenInfo Identifier: | 62511894 |
| NCBI Gene ID: | 4747 |
| NCBI Accession: | P07196.3 |
| UniProt Related Accession: | P07196 |
| Molecular Weight: | |
| NCBI Full Name: | Neurofilament light polypeptide |
| NCBI Synonym Full Names: | neurofilament light |
| NCBI Official Symbol: | NEFL |
| NCBI Official Synonym Symbols: | NFL; NF-L; NF68; CMT1F; CMT2E; CMTDIG; PPP1R110 |
| NCBI Protein Information: | neurofilament light polypeptide |
| UniProt Protein Name: | Neurofilament light polypeptide |
| UniProt Synonym Protein Names: | 68 kDa neurofilament protein; Neurofilament triplet L protein |
| Protein Family: | Neurofilament heavy polypeptide |
| UniProt Gene Name: | NEFL |
| UniProt Entry Name: | NFL_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NEFL antibody (CAB0257) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 120s. |
 | Immunohistochemistry of paraffin-embedded human breast cancer using NEFL antibody (CAB0257) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat kidney using NEFL antibody (CAB0257) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat brain using NEFL antibody (CAB0257) at dilution of 1:200 (40x lens). |
 | Immunofluorescence analysis of rat brain using NEFL Rabbit pAb (CAB0257) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
Related Products
Human NEFL ELISA Kit
Mouse Neurofilament light polypeptide (Nefl) ELISA Kit
