Anti-NDUFS4 항체 (CAB13519)

Antibody Name:	Anti-NDUFS4 Antibody
Antibody SKU:	CAB13519
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-175 of human NDUFS4 (NP_002486.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, HepG2, 293T, Mouse brain, Mouse bone marrow, Mouse liver, Rat brain, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-175 of human NDUFS4 (NP_002486.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAV SMSV VLRQ TLWR RRAV AVAA LSVS RVPT RSLR TSTW RLAQ DQTQ DTQL ITVD EKLD ITTL TGVP EEHI KTRK VRIF VPAR NNMQ SGVN NTKK WKME FDTR ERWE NPLM GWAS TADP LSNM VLTF STKE DAVS FAEK NGWS YDIE ERKV PKPK SKSY GANF SWNK RTRV STK
Gene ID:	4724
Uniprot:	O43181
Cellular Location:	Matrix side, Mitochondrion inner membrane, Peripheral membrane protein
Calculated MW:	20kDa
Observed MW:	20kDa

Synonyms:

NDUFS4, AQDQ, CI-18, CI-AQDQ

Background:

This gene encodes an nuclear-encoded accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (complex I, or NADH:ubiquinone oxidoreductase). Complex I removes electrons from NADH and passes them to the electron acceptor ubiquinone. Mutations in this gene can cause mitochondrial complex I deficiencies such as Leigh syndrome. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	NDUFS4: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Defects in NDUFS4 are a cause of mitochondrial complex I deficiency (MT-C1D). A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. Belongs to the complex I NDUFS4 subunit family.
UniProt Protein Details:	Protein type:EC 1.6.99.3; Oxidoreductase; EC 1.6.5.3; Mitochondrial; Energy Metabolism - oxidative phosphorylation Chromosomal Location of Human Ortholog: 5q11.1 Cellular Component: mitochondrion; mitochondrial inner membrane; mitochondrial respiratory chain complex I Molecular Function:NADH dehydrogenase (ubiquinone) activity Biological Process: mitochondrial respiratory chain complex I assembly; cellular metabolic process; regulation of protein amino acid phosphorylation; cAMP-mediated signaling; positive regulation of fibroblast proliferation; response to cAMP; mitochondrial electron transport, NADH to ubiquinone; cellular respiration; brain development Disease: Leigh Syndrome; Mitochondrial Complex I Deficiency
NCBI Summary:	This gene encodes an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), or NADH:ubiquinone oxidoreductase, the first multi-subunit enzyme complex of the mitochondrial respiratory chain. Complex I plays a vital role in cellular ATP production, the primary source of energy for many crucial processes in living cells. It removes electrons from NADH and passes them by a series of different protein-coupled redox centers to the electron acceptor ubiquinone. In well-coupled mitochondria, the electron flux leads to ATP generation via the building of a proton gradient across the inner membrane. Complex I is composed of at least 41 subunits, of which 7 are encoded by the mitochondrial genome and the remainder by nuclear genes. [provided by RefSeq, Jul 2008]
UniProt Code:	O43181
NCBI GenInfo Identifier:	3287881
NCBI Gene ID:	4724
NCBI Accession:	O43181.1
UniProt Secondary Accession:	O43181,Q9BS69,
UniProt Related Accession:	O43181
Molecular Weight:	175
NCBI Full Name:	NADH dehydrogenase
NCBI Synonym Full Names:	NADH dehydrogenase (ubiquinone) Fe-S protein 4, 18kDa (NADH-coenzyme Q reductase)
NCBI Official Symbol:	NDUFS4
NCBI Official Synonym Symbols:	AQDQ; CI-18
NCBI Protein Information:	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial; NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial; CI-AQDQ; CI-18 kDa; complex I-AQDQ; complex I-18 kDa; complex I 18kDa subunit; NADH-coenzyme Q reductase, 18-KD; NADH-ubiquinone oxidoreductase 18 kDa subunit; NADH dehydrogenase (ubiquinone) iron-sulfur protein 4; mitochondrial respiratory chain complex I (18-KD subunit)
UniProt Protein Name:	NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial
UniProt Synonym Protein Names:	Complex I-18 kDa; CI-18 kDa; Complex I-AQDQ; CI-AQDQ; NADH-ubiquinone oxidoreductase 18 kDa subunit
Protein Family:	NADH dehydrogenase [ubiquinone] iron-sulfur protein
UniProt Gene Name:	NDUFS4
UniProt Entry Name:	NDUS4_HUMAN

	Western blot analysis of extracts of various cell lines, using NDUFS4 antibody (CAB13519) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded rat kidney using NDUFS4 Rabbit pAb (CAB13519) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using NDUFS4 antibody (CAB13519). Blue: DAPI for nuclear staining.

Antibodies

Anti-NDUFS4 Antibody (CAB6390)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)