Anti-NDUFAF4 Antibody (CAB14345)
- SKU:
- CAB14345
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-NDUFAF4 Antibody |
| Antibody SKU: | CAB14345 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human NDUFAF4 (NP_054884.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, HeLa, K-562 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human NDUFAF4 (NP_054884.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MGAL VIRG IRNF NLEN RAER EISK MKPS VAPR HPST NSLL REQI SLYP EVKG EIAR KDEK LLSF LKDV YVDS KDPV SSLQ VKAA ETCQ EPKE FRLP KDHH FDMI NIKS IPKG KISI VEAL TLLN NHKL FPET WTAE KIMQ EYQL EQKD VNSL LKYF VTFE VEIF PPED KK |
| Gene ID: | 29078 |
| Uniprot: | Q9P032 |
| Cellular Location: | Lipid-anchor, Membrane, Mitochondrion |
| Calculated MW: | 20kDa |
| Observed MW: | 20kDa |
| Synonyms: | NDUFAF4, C6orf66, HRPAP20, HSPC125, My013, bA22L21.1 |
| Background: | NADH:ubiquinone oxidoreductase (complex I) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. This gene encodes a complex I assembly factor. Mutations in this gene are a cause of mitochondrial complex I deficiency. |
| UniProt Protein Function: | NDUFAF4: Involved in the assembly of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I). May be involved in cell proliferation and survival of hormone-dependent tumor cells. May be a regulator of breast tumor cell invasion. Defects in NDUFAF4 are a cause of mitochondrial complex I deficiency (MT-C1D). A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. Belongs to the NDUFAF4 family. |
| UniProt Protein Details: | Protein type:Mitochondrial; Apoptosis Chromosomal Location of Human Ortholog: 6q16.1 Cellular Component: mitochondrial inner membrane; mitochondrial membrane; mitochondrion Molecular Function:protein binding Biological Process: mitochondrial respiratory chain complex I assembly Disease: Mitochondrial Complex I Deficiency |
| NCBI Summary: | NADH:ubiquinone oxidoreductase (complex I) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. This gene encodes a complex I assembly factor. Mutations in this gene are a cause of mitochondrial complex I deficiency. [provided by RefSeq, Oct 2009] |
| UniProt Code: | Q9P032 |
| NCBI GenInfo Identifier: | 30912745 |
| NCBI Gene ID: | 29078 |
| NCBI Accession: | Q9P032.1 |
| UniProt Secondary Accession: | Q9P032,B2R4J5, |
| UniProt Related Accession: | Q9P032 |
| Molecular Weight: | 20,266 Da |
| NCBI Full Name: | NADH dehydrogenase |
| NCBI Synonym Full Names: | NADH:ubiquinone oxidoreductase complex assembly factor 4 |
| NCBI Official Symbol: | NDUFAF4 |
| NCBI Official Synonym Symbols: | My013; C6orf66; HRPAP20; HSPC125; bA22L21.1 |
| NCBI Protein Information: | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4 |
| UniProt Protein Name: | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4 |
| UniProt Synonym Protein Names: | Hormone-regulated proliferation-associated protein of 20 kDa |
| Protein Family: | NADH dehydrogenase |
| UniProt Gene Name: | NDUFAF4 |
| UniProt Entry Name: | NDUF4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NDUFAF4 antibody (CAB14345) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded Mouse heart using NDUFAF4 Rabbit pAb (CAB14345) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Rat heart using NDUFAF4 Rabbit pAb (CAB14345) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Human liver cancer using NDUFAF4 Rabbit pAb (CAB14345) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of HeLa cells using NDUFAF4 antibody (CAB14345) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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