Anti-NDUFAF1 Antibody (CAB15835)
- SKU:
- CAB15835
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| Antibody Name: | Anti-NDUFAF1 Antibody |
| Antibody SKU: | CAB15835 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-327 of human NDUFAF1 (NP_057097.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, 293T, A-549, Mouse heart, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-327 of human NDUFAF1 (NP_057097.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MALV HKLL RGTY FLRK FSKP TSAL YPFL GIRF AEYS SSLQ KPVA SPGK ASSQ RKTE GDLQ GDHQ KEVA LDIT SSEE KPDV SFDK AIRD EAIY HFRL LKDE IVDH WRGP EGHP LHEV LLEQ AKVV WQFR GKED LDKW TVTS DKTI GGRS EVFL KMGK NNQS ALLY GTLS SEAP QDGE STRS GYCA MISR IPRG AFER KMSY DWSQ FNTL YLRV RGDG RPWM VNIK EDTD FFQR TNQM YSYF MFTR GGPY WQEV KIPF SKFF FSNR GRIR DVQH ELPL DKIS SIGF TLAD KVDG PFFL EIDF IGVF TDPA HTEE FAYE NSPE LNPR LFK |
| Gene ID: | 51103 |
| Uniprot: | Q9Y375 |
| Cellular Location: | Mitochondrion |
| Calculated MW: | 37kDa |
| Observed MW: | 38kDa |
| Synonyms: | NDUFAF1, CGI-65, CGI65, CIA30 |
| Background: | This gene encodes a complex I assembly factor protein. Complex I (NADH-ubiquinone oxidoreductase) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. The encoded protein is required for assembly of complex I, and mutations in this gene are a cause of mitochondrial complex I deficiency. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 19. |
| UniProt Protein Function: | NDUFAF1: Chaperone protein involved in the assembly of the mitochondrial NADH:ubiquinone oxidoreductase complex (complex I). Belongs to the CIA30 family. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 15q11.2-q21.3 Cellular Component: cytoplasm; mitochondrial inner membrane Molecular Function:protein binding Biological Process: mitochondrial respiratory chain complex I assembly Disease: Mitochondrial Complex I Deficiency |
| NCBI Summary: | This gene encodes a complex I assembly factor protein. Complex I (NADH-ubiquinone oxidoreductase) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. The encoded protein is required for assembly of complex I, and mutations in this gene are a cause of mitochondrial complex I deficiency. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 19. [provided by RefSeq, Dec 2011] |
| UniProt Code: | Q9Y375 |
| NCBI GenInfo Identifier: | 21542405 |
| NCBI Gene ID: | 51103 |
| NCBI Accession: | Q9Y375.2 |
| UniProt Secondary Accession: | Q9Y375,Q9BVZ5, |
| UniProt Related Accession: | Q9Y375 |
| Molecular Weight: | 37,764 Da |
| NCBI Full Name: | Complex I intermediate-associated protein 30, mitochondrial |
| NCBI Synonym Full Names: | NADH:ubiquinone oxidoreductase complex assembly factor 1 |
| NCBI Official Symbol: | NDUFAF1 |
| NCBI Official Synonym Symbols: | CGI65; CIA30; CGI-65 |
| NCBI Protein Information: | complex I intermediate-associated protein 30, mitochondrial |
| UniProt Protein Name: | Complex I intermediate-associated protein 30, mitochondrial |
| UniProt Synonym Protein Names: | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 1 |
| UniProt Gene Name: | NDUFAF1 |
| UniProt Entry Name: | CIA30_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NDUFAF1 antibody (CAB15835) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 35s. |
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