Anti-NDUFA9 Antibody (CAB3196)
- SKU:
- CAB3196
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-NDUFA9 Antibody |
| Antibody SKU: | CAB3196 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human NDUFA9 (NP_004993.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | LO2, BT-474, HL-60, HT-29, Mouse kidney, Mouse heart, Mouse brain, Mouse liver, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human NDUFA9 (NP_004993.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAAA AQSR VVRV LSMS RSAI TAIA TSVC HGPP CRQL HHAL MPHG KGGR SSVS GIVA TVFG ATGF LGRY VVNH LGRM GSQV IIPY RCDK YDIM HLRP MGDL GQLL FLEW DARD KDSI RRVV QHSN VVIN LIGR DWET KNFD FEDV FVKI PQAI AQLS KEAG VEKF IHVS HLNA NIKS SSRY LRNK AVGE KVVR DAFP EAII VKPS DIFG REDR FLNS FASM HRFG PIPL GSLG WKTV KQPV YVVD VSKG IVNA VKDP DANG KSFA FVGP SR |
| Gene ID: | 4704 |
| Uniprot: | Q16795 |
| Cellular Location: | Mitochondrion matrix |
| Calculated MW: | 42kDa |
| Observed MW: | 38kDa |
| Synonyms: | NDUFA9, CC6, CI-39k, CI39k, NDUFS2L, SDR22E1 |
| Background: | The encoded protein is a subunit of the hydrophobic protein fraction of the NADH:ubiquinone oxidoreductase (complex I), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane. A pseudogene has been identified on chromosome 12. |
| UniProt Protein Function: | NUEM: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Belongs to the complex I NDUFA9 subunit family. |
| UniProt Protein Details: | Protein type:Oxidoreductase; Mitochondrial; EC 1.6.99.3; EC 1.6.5.3; Energy Metabolism - oxidative phosphorylation Chromosomal Location of Human Ortholog: 12p13.3 Cellular Component: mitochondrial matrix; mitochondrial membrane; mitochondrial respiratory chain complex I; mitochondrion; nucleoplasm; nucleus Molecular Function:NADH dehydrogenase activity; protein binding; protein complex binding Biological Process: mitochondrial electron transport, NADH to ubiquinone; mitochondrial respiratory chain complex I assembly Disease: Leigh Syndrome |
| NCBI Summary: | The encoded protein is a subunit of the hydrophobic protein fraction of the NADH:ubiquinone oxidoreductase (complex I), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane. A pseudogene has been identified on chromosome 12. [provided by RefSeq, May 2010] |
| UniProt Code: | Q16795 |
| NCBI GenInfo Identifier: | 2833280 |
| NCBI Gene ID: | 4704 |
| NCBI Accession: | Q16795.2 |
| UniProt Secondary Accession: | Q16795,Q14076, Q2NKX0, |
| UniProt Related Accession: | Q16795 |
| Molecular Weight: | 42,510 Da |
| NCBI Full Name: | NADH dehydrogenase |
| NCBI Synonym Full Names: | NADH:ubiquinone oxidoreductase subunit A9 |
| NCBI Official Symbol: | NDUFA9 |
| NCBI Official Synonym Symbols: | CC6; CI39k; CI-39k; NDUFS2L; SDR22E1 |
| NCBI Protein Information: | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial |
| UniProt Protein Name: | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial |
| UniProt Synonym Protein Names: | Complex I-39kD; CI-39kD; NADH-ubiquinone oxidoreductase 39 kDa subunit |
| UniProt Gene Name: | NDUFA9 |
| UniProt Entry Name: | NDUA9_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NDUFA9 antibody (CAB3196) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
Related Products
