Anti-NDRG1 Antibody (RACO0144)
- SKU:
- RACO0144
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- IF
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| Product Name: | NDRG1 Antibody |
| Product SKU: | RACO0144 |
| Size: | 50ul |
| Host Species: | Human |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200 |
| Species Reactivity: | Human, Mouse |
| Immunogen: | A synthesized peptide derived from human NDRG1 |
| Form: | Liquid |
| Storage Buffer: | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Purification Method: | Affinity-chromatography |
| Clonality: | Monoclonal |
| Isotype: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| Background: | Stress-responsive protein involved in hormone responses, cell growth, and differentiation. Acts as a tumor suppressor in many cell types. Necessary but not sufficient for p53/TP53-mediated caspase activation and apoptosis. Has a role in cell trafficking, notably of the Schwann cell, and is necessary for the maintenance and development of the peripheral nerve myelin sheath. Required for vesicular recycling of CDH1 and TF. May also function in lipid trafficking. Protects cells from spindle disruption damage. Functions in p53/TP53-dependent mitotic spindle checkpoint. Regulates microtubule dynamics and maintains euploidy. |
| Synonyms: | Protein NDRG1, Differentiation-related gene 1 protein, DRG-1, N-myc downstream-regulated gene 1 protein, Nickel-specific induction protein Cap43, Reducing agents and tunicamycin-responsive protein, RTP, Rit42, NDRG1, CAP43, DRG1, RTP |
 | IHC image of RACO0144 diluted at 1:71.6666666666667 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of Hela cells with RACO0144 at 1:23, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
 | Western Blot. Positive WB detected in Hela whole cell lysate 293T whole cell lysate PC3 whole cell lysate U87 whole cell lysate Mouse kidney tissue. All lanes NDRG1 antibody at 0.7µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 43, 36, 34 KDa. Observed band size: 43 KDa. |
| UniProt Protein Function: | NDRG1: a hypoxia-inducible anti-apoptotic protein. Promotes proliferation and correlates with invasion, metastasis and poor prognosis in many cancers. Is involved in growth arrest and cell differentiation during development. A ubiquitous Rab4a effector protein that modulates angiogenesis and is involved in vesicular recycling of E-cadherin. Colocalizes with transferrin during the recycling in cells. NDRG1 knockdown delays the recycling rate of transferrin, while its overexpression increases the rate of transferrin recycling. Interacts with SIRT1/p53 signaling to attenuate hypoxic injury in human trophoblasts. Its expression is induced by an elevation of free intracellular calcium ion caused by nickel ion exposure. Highly expressed in placental membranes, prostate, kidney, small intestine, ovary, peripheral nerves and Schwann cells. Defects in NDRG1 are the cause of Charcot-Marie-Tooth disease type 4D (CMT4D). |
| UniProt Protein Details: | Protein type:Cell development/differentiation; Vesicle Chromosomal Location of Human Ortholog: 8q24.3 Cellular Component: microtubule cytoskeleton; microtubule; centrosome; cell-cell adherens junction; recycling endosome membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; cytosol; nucleus Molecular Function:protein binding; gamma-tubulin binding; cadherin binding; microtubule binding; Rab GTPase binding Biological Process: response to metal ion; DNA damage response, signal transduction by p53 class mediator; myelin maintenance in the peripheral nervous system; mast cell activation Disease: Charcot-marie-tooth Disease, Type 4d |
| NCBI Summary: | This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. The encoded protein is necessary for p53-mediated caspase activation and apoptosis. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012] |
| UniProt Code: | Q92597 |
| NCBI GenInfo Identifier: | 6166568 |
| NCBI Gene ID: | 10397 |
| NCBI Accession: | Q92597.1 |
| UniProt Secondary Accession: | Q92597,O15207, Q6IBG2, Q9NYR6, Q9UK29, B3KR80, B7Z446 |
| UniProt Related Accession: | Q92597 |
| Molecular Weight: | 394 |
| NCBI Full Name: | Protein NDRG1 |
| NCBI Synonym Full Names: | N-myc downstream regulated 1 |
| NCBI Official Symbol: | NDRG1Â Â |
| NCBI Official Synonym Symbols: | GC4; RTP; DRG1; NDR1; NMSL; TDD5; CAP43; CMT4D; DRG-1; HMSNL; RIT42; TARG1; PROXY1Â Â |
| NCBI Protein Information: | protein NDRG1; protein regulated by oxygen-1; differentiation-related gene 1 protein; nickel-specific induction protein Cap43; N-myc downstream-regulated gene 1 protein; reducing agents and tunicamycin-responsive protein |
| UniProt Protein Name: | Protein NDRG1 |
| UniProt Synonym Protein Names: | Differentiation-related gene 1 protein; DRG-1; N-myc downstream-regulated gene 1 protein; Nickel-specific induction protein Cap43; Reducing agents and tunicamycin-responsive protein; RTP; Rit42 |
| Protein Family: | Protein |
| UniProt Gene Name: | NDRG1Â Â |
| UniProt Entry Name: | NDRG1_HUMAN |
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