Anti-NDE1 Antibody (CAB7112)
- SKU:
- CAB7112
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| Antibody Name: | Anti-NDE1 Antibody |
| Antibody SKU: | CAB7112 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human NDE1 (NP_060138.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, HepG2, SKOV3, MCF7, A673, Mouse heart, Mouse lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human NDE1 (NP_060138.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MEDS GKTF SSEE EEAN YWKD LAMT YKQR AENT QEEL REFQ EGSR EYEA ELET QLQQ IETR NRDL LSEN NRLR MELE TIKE KFEV QHSE GYRQ ISAL EDDL AQTK AIKD QL |
| Gene ID: | 54820 |
| Uniprot: | Q9NXR1 |
| Cellular Location: | Chromosome, Cleavage furrow, Cytoplasm, centromere, centrosome, cytoskeleton, kinetochore, microtubule organizing center, spindle |
| Calculated MW: | 37kDa/38kDa |
| Observed MW: | 39kDa |
| Synonyms: | NDE1, HOM-TES-87, LIS4, MHAC, NDE, NUDE, NUDE1 |
| Background: | This gene encodes a member of the nuclear distribution E (NudE) family of proteins. The encoded protein is localized at the centrosome and interacts with other centrosome components as part of a multiprotein complex that regulates dynein function. This protein plays an essential role in microtubule organization, mitosis and neuronal migration. Mutations in this gene cause lissencephaly 4, a disorder characterized by lissencephaly, severe brain atrophy, microcephaly, and severe mental retardation. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | NUDE1: a microtubule-associated protein required for centrosome duplication, and the formation and function of the mitotic spindle. Associated with the centrosome, kinetochore, and spindle. Specifically phosphorylated during M phase, inducing its association with mitotic spindles. Appears to participate in dynein-mediated transport of kinetochore proteins to spindle poles along microtubules. Interacts with gamma tubulin, dynactin, CEP110, dynein,TCP1. PAFAH1B1, PCM-1 and PCNT. Concentrates at the plus ends of microtubules coincident with kinetochores in metaphase and anaphase, and at the cleavage furrow during cytokinesis.Two alternatively spliced human isoforms have been described. |
| UniProt Protein Details: | Protein type:Microtubule-binding; Cell cycle regulation; Cytoskeletal Chromosomal Location of Human Ortholog: 16p13.11 Cellular Component: kinetochore; centrosome; kinesin complex; microtubule; membrane; spindle pole centrosome; cytosol; cleavage furrow Molecular Function:protein domain specific binding; identical protein binding; protein binding; microtubule binding Biological Process: cell migration; organelle organization and biogenesis; establishment of chromosome localization; neuron migration; microtubule nucleation; chromosome segregation; mitotic centrosome separation; centrosome localization; establishment of mitotic spindle orientation; cell division; neuroblast proliferation; vesicle transport along microtubule; mitotic cell cycle; cerebral cortex development; G2/M transition of mitotic cell cycle; centrosome duplication Disease: Lissencephaly 4; Microhydranencephaly |
| UniProt Code: | Q9NXR1 |
| NCBI GenInfo Identifier: | 108860813 |
| NCBI Gene ID: | |
| NCBI Accession: | Q9NXR1.2 |
| Molecular Weight: | 37kDa; 38kDa |
| NCBI Full Name: | Nuclear distribution protein nudE homolog 1 |
| UniProt Protein Name: | Nuclear distribution protein nudE homolog 1 |
| Protein Family: | Nuclear distribution protein |
| UniProt Gene Name: | NDE1 |
| UniProt Entry Name: | NDE1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NDE1 antibody (CAB7112) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat brain using NDE1 antibody (CAB7112) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat spleen using NDE1 antibody (CAB7112) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human breast cancer using NDE1 antibody (CAB7112) at dilution of 1:100 (40x lens). |
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