Anti-NASP Antibody (CAB6938)
- SKU:
- CAB6938
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| Antibody Name: | Anti-NASP Antibody |
| Antibody SKU: | CAB6938 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 180-449 of human NASP (NP_689511.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SW620, HL-60, 293T |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 180-449 of human NASP (NP_689511.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | WDML DLAK IIFK RQET KEAQ LYAA QAHL KLGE VSVE SENY VQAV EEFQ SCLN LQEQ YLEA HDRL LAET HYQL GLAY GYNS QYDE AVAQ FSKS IEVI ENRM AVLN EQVK EAEG SSAE YKKE IEEL KELL PEIR EKIE DAKE SQRS GNVA ELAL KATL VESS TSGF TPGG GGSS VSMI ASRK PTDG ASSS NCVT DISH LVRK KRKP EEES PRKD DAKK AKQE PEVN GGSG DAVP SGNE VSEN MEEE AENQ AESR AAVE GTVE AGAT VEST AC |
| Gene ID: | 4678 |
| Uniprot: | P49321 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 48kDa/78kDa/85kDa/86kDa |
| Observed MW: | 85kDa |
| Synonyms: | NASP, FLB7527, HMDRA1, PRO1999 |
| Background: | This gene encodes a H1 histone binding protein that is involved in transporting histones into the nucleus of dividing cells. Multiple isoforms are encoded by transcript variants of this gene. The somatic form is expressed in all mitotic cells, is localized to the nucleus, and is coupled to the cell cycle. The testicular form is expressed in embryonic tissues, tumor cells, and the testis. In male germ cells, this protein is localized to the cytoplasm of primary spermatocytes, the nucleus of spermatids, and the periacrosomal region of mature spermatozoa. |
| UniProt Protein Function: | NASP: Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and H1 linker histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA. Belongs to the NASP family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cell cycle regulation Chromosomal Location of Human Ortholog: 1p34.1 Cellular Component: nucleoplasm; protein complex; cytoplasm; nuclear chromatin; nucleus Molecular Function:histone binding; Hsp90 protein binding Biological Process: cell proliferation; blastocyst development; protein transport; DNA replication-independent nucleosome assembly; histone exchange; cell cycle; DNA replication; DNA replication-dependent nucleosome assembly |
| UniProt Code: | P49321 |
| NCBI GenInfo Identifier: | 23503077 |
| NCBI Gene ID: | |
| NCBI Accession: | P49321.2 |
| Molecular Weight: | ~ 85kDa |
| NCBI Full Name: | Nuclear autoantigenic sperm protein |
| UniProt Protein Name: | Nuclear autoantigenic sperm protein |
| Protein Family: | NASP-related protein |
| UniProt Gene Name: | NASP |
| UniProt Entry Name: | NASP_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NASP antibody (CAB6938) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat spleen using NASP Antibody (CAB6938) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human brain astrocytoma using NASP Antibody (CAB6938) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse spleen using NASP Antibody (CAB6938) at dilution of 1:100 (40x lens). |
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