Anti-NANS Antibody (CAB19875)[KO Validated]

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SKU:
CAB19875
Product type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
Frequently bought together:

Description

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Antibody Name:Anti-NANS Antibody [KO Validated]
Antibody SKU:CAB19875
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant protein of human NANS.
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:293T
Immunogen:Recombinant protein of human NANS.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:54187
Uniprot:Q9NR45
Cellular Location:
Calculated MW:40kDa
Observed MW:40KDa
Anti-NANS Antibody [KO Validated] (CAB19875)Western blot analysis of extracts from normal (control) and NANS knockout (KO) 293T cells, using NANS antibody (CAB19875) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Synonyms:NANS, HEL-S-100, SAS, SEMDCG, SEMDG
Background:This gene encodes an enzyme that functions in the biosynthetic pathways of sialic acids. In vitro, the encoded protein uses N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), respectively; however, it exhibits much higher activity toward the Neu5Ac phosphate product. In insect cells, expression of this gene results in Neu5Ac and KDN production. This gene is related to the E. coli sialic acid synthase gene neuB, and it can partially restore sialic acid synthase activity in an E. coli neuB-negative mutant.
UniProt Protein Function:NANS: Produces N-acetylneuraminic acid (Neu5Ac) and 2-keto-3- deoxy-D-glycero-D-galacto-nononic acid (KDN). Can also use N- acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of Neu5Ac and KDN, respectively.
UniProt Protein Details:

Protein type:Carbohydrate Metabolism - amino sugar and nucleotide sugar; EC 2.5.1.57; Transferase; EC 2.5.1.56

Chromosomal Location of Human Ortholog: 9p24.1-p23

Cellular Component: cytoplasm; cytosol

Molecular Function:N-acylneuraminate-9-phosphate synthase activity; N-acetylneuraminate synthase activity; N-acylneuraminate cytidylyltransferase activity

Biological Process: cellular protein metabolic process; dolichol-linked oligosaccharide biosynthetic process; lipopolysaccharide biosynthetic process; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification

NCBI Summary:This gene encodes an enzyme that functions in the biosynthetic pathways of sialic acids. In vitro, the encoded protein uses N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), respectively; however, it exhibits much higher activity toward the Neu5Ac phosphate product. In insect cells, expression of this gene results in Neu5Ac and KDN production. This gene is related to the E. coli sialic acid synthase gene neuB, and it can partially restore sialic acid synthase activity in an E. coli neuB-negative mutant. [provided by RefSeq, Jul 2008]
UniProt Code:Q9NR45
NCBI GenInfo Identifier:20978759
NCBI Gene ID:54187
NCBI Accession:Q9NR45.2
UniProt Secondary Accession:Q9NR45,Q8WUV9, Q9BWS6, Q9NVD4, B2RE98,
UniProt Related Accession:Q9NR45
Molecular Weight:359
NCBI Full Name:Sialic acid synthase
NCBI Synonym Full Names:N-acetylneuraminic acid synthase
NCBI Official Symbol:NANS  
NCBI Official Synonym Symbols:SAS; HEL-S-100  
NCBI Protein Information:sialic acid synthase; N-acetylneuraminate synthase; sialic acid phosphate synthase; epididymis secretory protein Li 100; N-acetylneuraminate-9-phosphate synthase; N-acetylneuraminic acid phosphate synthase
UniProt Protein Name:Sialic acid synthase
UniProt Synonym Protein Names:N-acetylneuraminate synthase (EC:2.5.1.56); N-acetylneuraminate-9-phosphate synthase (EC:2.5.1.57); N-acetylneuraminic acid phosphate synthase; N-acetylneuraminic acid synthase
Protein Family:Sialic acid synthase
UniProt Gene Name:NANS  
UniProt Entry Name:SIAS_HUMAN

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