Anti-NAE1 Antibody (CAB4254)
- SKU:
- CAB4254
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Death
Frequently bought together:
Description
| Antibody Name: | Anti-NAE1 Antibody |
| Antibody SKU: | CAB4254 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human NAE1 (NP_003896.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:1000 - 1:4000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SH-SY5Y, BT-474, HL-60, SW620, NIH/3T3, Mouse brain, Mouse lung, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human NAE1 (NP_003896.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAQL GKLL KEQK YDRQ LRLW GDHG QEAL ESAH VCLI NATA TGTE ILKN LVLP GIGS FTII DGNQ VSGE DAGN NFFL QRSS IGKN RAEA AMEF LQEL NSDV SGSF VEES PENL LDND PSFF CRFT VVVA TQLP ESTS LRLA DVLW NSQI PLLI CRTY GLVG YMRI IIKE HPVI ESHP DNAL EDLR LDKP FPEL REHF QSYD LDHM EKKD HSHT PWIV IIAK |
| Gene ID: | 8883 |
| Uniprot: | Q13564 |
| Cellular Location: | Cell membrane |
| Calculated MW: | 50kDa/59kDa/60kDa |
| Observed MW: | 60kDa |
| Synonyms: | NAE1, A-116A10.1, APPBP1, HPP1, ula-1 |
| Background: | The protein encoded by this gene binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. In addition, the encoded protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein. This protein is required for cell cycle progression through the S/M checkpoint. Three transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | APPBP1: Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation. Belongs to the ubiquitin-activating E1 family. ULA1 subfamily. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 16q22 Cellular Component: cytoplasm; plasma membrane; nucleus; cytosol Molecular Function:NEDD8 activating enzyme activity; protein binding; protein heterodimerization activity; ubiquitin protein ligase binding Biological Process: regulation of apoptosis; neuron apoptosis; protein neddylation; regulation of neuron apoptosis; mitotic cell cycle DNA replication checkpoint; signal transduction |
| NCBI Summary: | The protein encoded by this gene binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. In addition, the encoded protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein. This protein is required for cell cycle progression through the S/M checkpoint. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q13564 |
| NCBI GenInfo Identifier: | 50400302 |
| NCBI Gene ID: | 8883 |
| NCBI Accession: | Q13564.1 |
| UniProt Related Accession: | Q13564 |
| Molecular Weight: | |
| NCBI Full Name: | NEDD8-activating enzyme E1 regulatory subunit |
| NCBI Synonym Full Names: | NEDD8 activating enzyme E1 subunit 1 |
| NCBI Official Symbol: | NAE1 |
| NCBI Official Synonym Symbols: | HPP1; ula-1; APPBP1; A-116A10.1 |
| NCBI Protein Information: | NEDD8-activating enzyme E1 regulatory subunit |
| UniProt Protein Name: | NEDD8-activating enzyme E1 regulatory subunit |
| UniProt Synonym Protein Names: | Amyloid beta precursor protein-binding protein 1, 59 kDa; APP-BP1; Amyloid protein-binding protein 1; Proto-oncogene protein 1 |
| UniProt Gene Name: | NAE1 |
| UniProt Entry Name: | ULA1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NAE1 antibody (CAB4254) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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