Anti-NAD kinase Antibody (CAB9111)
- SKU:
- CAB9111
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-NAD kinase Antibody |
| Antibody SKU: | CAB9111 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human NADK (NP_075394.3). |
| Application: | WB |
| Recommended Dilution: | WB 1:1000 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, Mouse liver, Mouse brain, Rat liver, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human NADK (NP_075394.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MEME QEKM TMNK ELSP DAAA YCCS ACHG DETW SYNH PIRG RAKS RSLS ASPA LGST KEFR RTRS LHGP CPVT TFGP KACV LQNP QTIM HIQD PASQ RLTW NKSP KSVL VIKK MRDA SLLQ PFKE LCTH LMEE NMIV YVEK KVLE DPAI ASDE SFGA VKKK FCTF REDY DDIS NQID FIIC |
| Gene ID: | 65220 |
| Uniprot: | O95544 |
| Cellular Location: | |
| Calculated MW: | 46kDa/49kDa/63kDa |
| Observed MW: | 49kDa |
| Synonyms: | NADK, dJ283E3.1 |
| Background: |
| UniProt Protein Function: | NADK: Belongs to the NAD kinase family |
| UniProt Protein Details: | Protein type:Kinase, other; EC 2.7.1.23; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide Chromosomal Location of Human Ortholog: 1p36.33 Cellular Component: cytosol Molecular Function:ATP binding; metal ion binding; NAD+ kinase activity; protein binding Biological Process: ATP metabolic process; NAD metabolic process; NADP biosynthetic process; phosphorylation; vitamin metabolic process; water-soluble vitamin metabolic process |
| NCBI Summary: | NADK catalyzes the transfer of a phosphate group from ATP to NAD to generate NADP, which in its reduced form acts as an electron donor for biosynthetic reactions (Lerner et al., 2001 [PubMed 11594753]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | O95544 |
| NCBI GenInfo Identifier: | 312222779 |
| NCBI Gene ID: | 65220 |
| NCBI Accession: | NP_001185922.1 |
| UniProt Secondary Accession: | O95544,Q5QPS4, Q9H2P2, Q9H931, A6NNN3, A8K296, B7Z434 F5GXR5, |
| UniProt Related Accession: | O95544 |
| Molecular Weight: | Calculated MW: 46kDa/49kDa/63kDaObserved MW: 49kDa |
| NCBI Full Name: | NAD kinase isoform 1 |
| NCBI Synonym Full Names: | NAD kinase |
| NCBI Official Symbol: | NADK |
| NCBI Official Synonym Symbols: | dJ283E3.1 |
| NCBI Protein Information: | NAD kinase |
| UniProt Protein Name: | NAD kinase |
| UniProt Synonym Protein Names: | Poly(P)/ATP NAD kinase |
| Protein Family: | NAD kinase |
| UniProt Gene Name: | NADK |
| UniProt Entry Name: | NADK_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NADK antibody (CAB9111) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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