Anti-N-WASP Antibody (CAB2576)

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SKU:
CAB2576
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle
Frequently bought together:

Description

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Antibody Name:Anti-N-WASP Antibody
Antibody SKU:CAB2576
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human N-WASP (NP_003932.3).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, U-87MG, A-549, Mouse brain, Mouse lung, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human N-WASP (NP_003932.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSSV QQQP PPPR RVTN VGSL LLTP QENE SLFT FLGK KCVT MSSA VVQL YAAD RNCM WSKK CSGV ACLV KDNP QRSY FLRI FDIK DGKL LWEQ ELYN NFVY NSPR GYFH TFAG DTCQ VALN FANE EEAK KFRK AVTD LLGR RQRK SEKR RDPP NGPN LPMA TVDI KNPE ITTN RFYG PQVN NISH TKEK KKGK AKKK RLTK ADIG TPSN FQHI GHVG WDPN TGFD LNNL DPEL KNLF DMCG ISEA QLKD RE
Gene ID:8976
Uniprot:O00401
Cellular Location:Cytoplasm, Nucleus, cytoskeleton
Calculated MW:54kDa
Observed MW:65kDa
Synonyms:WASL, N-WASP, NWASP, WASPB
Background:This gene encodes a member of the Wiskott-Aldrich syndrome (WAS) protein family. Wiskott-Aldrich syndrome proteins share similar domain structure, and associate with a variety of signaling molecules to alter the actin cytoskeleton. The encoded protein is highly expressed in neural tissues, and interacts with several proteins involved in cytoskeletal organization, including cell division control protein 42 (CDC42) and the actin-related protein-2/3 (ARP2/3) complex. The encoded protein may be involved in the formation of long actin microspikes, and in neurite extension.
UniProt Protein Function:N-WASP: Regulates actin polymerization by stimulating the actin- nucleating activity of the Arp2/3 complex. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression. Binds actin and the Arp2/3 complex. Interacts with CDC42. Binds to SH3 domains of GRB2. Interacts with the C-terminal SH3 domain of DNMBP. Interacts with SNX9. Interacts with the WW domains of PRPF40A/FBP11. Interacts with PTK2/FAK1. Interacts with NOSTRIN. Interacts with Shigella flexneri protein IcsA. The interaction with IcsA enhances the affinity of WASL for Arp2/3, thus assembling a tight complex which has maximal activity in actin assembly. Interacts with E.coli effector protein EspF(U). Identified in a complex containing at least WASL, BAIAP2L1 and E.coli EspF(U). Binds to TNK2.
UniProt Protein Details:

Protein type:Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 7q31.3

Cellular Component: lamellipodium; cytoplasmic membrane-bound vesicle; plasma membrane; actin cap; nucleus; cytosol; actin cytoskeleton

Molecular Function:protein binding; actin binding

Biological Process: positive regulation of filopodium formation; axon guidance; mitosis; transcription, DNA-dependent; vesicle organization and biogenesis; nitric oxide metabolic process; spindle localization; vesicle transport along actin filament; regulation of protein localization; regulation of transcription, DNA-dependent; response to bacterium; cell division; actin polymerization and/or depolymerization; ephrin receptor signaling pathway; innate immune response; protein complex assembly; regulation of nitric-oxide synthase activity; cell motility; membrane budding

NCBI Summary:This gene encodes a member of the Wiskott-Aldrich syndrome (WAS) protein family. Wiskott-Aldrich syndrome proteins share similar domain structure, and associate with a variety of signaling molecules to alter the actin cytoskeleton. The encoded protein is highly expressed in neural tissues, and interacts with several proteins involved in cytoskeletal organization, including cell division control protein 42 (CDC42) and the actin-related protein-2/3 (ARP2/3) complex. The encoded protein may be involved in the formation of long actin microspikes, and in neurite extension. [provided by RefSeq, Jul 2013]
UniProt Code:O00401
NCBI GenInfo Identifier:62291053
NCBI Gene ID:8976
NCBI Accession:O00401.2
UniProt Secondary Accession:O00401,Q7Z746, A1JUI9,
UniProt Related Accession:O00401
Molecular Weight:505
NCBI Full Name:Neural Wiskott-Aldrich syndrome protein
NCBI Synonym Full Names:Wiskott-Aldrich syndrome-like
NCBI Official Symbol:WASL  
NCBI Official Synonym Symbols:NWASP; N-WASP  
NCBI Protein Information:neural Wiskott-Aldrich syndrome protein
UniProt Protein Name:Neural Wiskott-Aldrich syndrome protein
Protein Family:Neural Wiskott-Aldrich syndrome protein
UniProt Gene Name:WASL  
UniProt Entry Name:WASL_HUMAN
Anti-N-WASP Antibody (CAB2576)Western blot analysis of extracts of various cell lines, using N-WASP antibody (CAB2576) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-N-WASP Antibody (CAB2576)Immunohistochemistry of paraffin-embedded rat brain using N-WASP antibody (CAB2576) at dilution of 1:100 (40x lens).
Anti-N-WASP Antibody (CAB2576)Immunohistochemistry of paraffin-embedded human colon carcinoma using N-WASP antibody (CAB2576) at dilution of 1:100 (40x lens).
Anti-N-WASP Antibody (CAB2576)Immunohistochemistry of paraffin-embedded mouse heart using N-WASP antibody (CAB2576) at dilution of 1:100 (40x lens).
Anti-N-WASP Antibody (CAB2576)Immunofluorescence analysis of C6 cells using N-WASP Polyclonal Antibody (CAB2576) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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