Anti-MYL2 Antibody (CAB14188)
- SKU:
- CAB14188
- Product type:
- Antibody
- Reactivity:
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-MYL2 Antibody |
| Antibody SKU: | CAB14188 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant protein of human MYL2 |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse heart, Mouse skeletal muscle, Rat heart, Rat skeletal muscle |
| Immunogen: | Recombinant protein of human MYL2 |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 4633 |
| Uniprot: | P10916 |
| Cellular Location: | A band, Cytoplasm, myofibril, sarcomere |
| Calculated MW: | 18kDa |
| Observed MW: | 19kDa |
| Synonyms: | MYL2, CMH10, MLC-2s/v, MLC2 |
| Background: | Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. |
| UniProt Protein Function: | MRLC2V: myosin regulatory light chain 2, ventricular/cardiac muscle isoform. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. |
| UniProt Protein Details: | Protein type:Contractile Chromosomal Location of Human Ortholog: 12q24.11 Cellular Component: sarcomere; cytoskeleton; myofibril; cytosol; myosin complex; A band; actin cytoskeleton Molecular Function:actin monomer binding; protein binding; structural constituent of muscle; calcium ion binding; myosin heavy chain binding Biological Process: muscle cell fate specification; heart contraction; regulation of striated muscle contraction; ventricular cardiac muscle morphogenesis; cardiac myofibril assembly; negative regulation of cell growth; muscle fiber development; muscle filament sliding; cardiac muscle contraction; post-embryonic development Disease: Cardiomyopathy, Familial Hypertrophic, 10 |
| NCBI Summary: | Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P10916 |
| NCBI GenInfo Identifier: | 6166556 |
| NCBI Gene ID: | 4633 |
| NCBI Accession: | P10916.3 |
| UniProt Secondary Accession: | P10916,Q16123, |
| UniProt Related Accession: | P10916 |
| Molecular Weight: | 166 |
| NCBI Full Name: | Myosin regulatory light chain 2, ventricular/cardiac muscle isoform |
| NCBI Synonym Full Names: | myosin, light chain 2, regulatory, cardiac, slow |
| NCBI Official Symbol: | MYL2 |
| NCBI Official Synonym Symbols: | MLC2; CMH10 |
| NCBI Protein Information: | myosin regulatory light chain 2, ventricular/cardiac muscle isoform; MLC-2; MLC-2v; RLC of myosin; myosin light chain 2; regulatory light chain of myosin; cardiac ventricular myosin light chain 2; slow cardiac myosin regulatory light chain 2; myosin, light polypeptide 2, regulatory, cardiac, slow |
| UniProt Protein Name: | Myosin regulatory light chain 2, ventricular/cardiac muscle isoform |
| Protein Family: | Myosin regulatory |
| UniProt Gene Name: | MYL2 |
| UniProt Entry Name: | MLRV_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MYL2 antibody (CAB14188) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of rat heart using MYL2 Rabbit pAb (CAB14188) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of mouse heart using MYL2 Rabbit pAb (CAB14188) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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