Anti-Myelin Basic protein Antibody (CAB1664)
- SKU:
- CAB1664
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-Myelin Basic protein Antibody |
| Antibody SKU: | CAB1664 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-197 of human Myelin Basic protein (NP_001020271.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | NB4, SH-SY5Y, HL-60, HT-29, PC-3, PC-12, BT-474, Mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-197 of human Myelin Basic protein (NP_001020271.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MGNH AGKR ELNA EKAS TNSE TNRG ESEK KRNL GELS RTTS EDNE VFGE ADAN QNNG TSSQ DTAV TDSK RTAD PKNA WQDA HPAD PGSR PHLI RLFS RDAP GRED NTFK DRPS ESDE LQTI QEDS AATS ESLD VMAS QKRP SQRH GSKY LATA STMD HARH GFLP RHRD TGIL DSIG RFFG GDRG APKR GSGK VSSE E |
| Gene ID: | 4155 |
| Uniprot: | P02686 |
| Cellular Location: | Cytoplasmic side, Myelin membrane, Nucleus, Peripheral membrane protein |
| Calculated MW: | 17kDa/18kDa/20kDa/21kDa/33kDa |
| Observed MW: | 17kDa/33kDa/40kDa |
| Synonyms: | MBP |
| Background: | The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called 'Golli-MBP') that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. |
| UniProt Protein Function: | MBP: The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non- classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T- cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation. Homodimer. Isoform 3 exists as a homodimer. MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system. Belongs to the myelin basic protein family. 6 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cytoskeletal Chromosomal Location of Human Ortholog: 18q23 Cellular Component: compact myelin; internode region of axon; cell soma; plasma membrane; nucleus Molecular Function:protein binding; protease binding; structural constituent of myelin sheath Biological Process: myelination; response to mercury ion; synaptic transmission; substantia nigra development; central nervous system development; response to toxin; negative regulation of axonogenesis; immune response; axon ensheathment; response to progesterone stimulus; aging |
| NCBI Summary: | The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P02686 |
| NCBI GenInfo Identifier: | 17378805 |
| NCBI Gene ID: | 4155 |
| NCBI Accession: | P02686.3 |
| UniProt Secondary Accession: | P02686,Q15337, Q15338, Q15339, Q15340, A4FU54, A6NI84 A8MY86, A8MYL4, B3KY66, B7ZKS2, B7ZKS4, |
| UniProt Related Accession: | P02686 |
| Molecular Weight: | |
| NCBI Full Name: | Myelin basic protein |
| NCBI Synonym Full Names: | myelin basic protein |
| NCBI Official Symbol: | MBP |
| NCBI Protein Information: | Golli-MBP; myelin basic protein; myelin A1 protein; myelin membrane encephalitogenic protein |
| UniProt Protein Name: | Myelin basic protein |
| UniProt Synonym Protein Names: | Myelin A1 protein; Myelin membrane encephalitogenic protein |
| Protein Family: | Myelin basic protein |
| UniProt Gene Name: | MBP |
| UniProt Entry Name: | MBP_HUMAN |
 | Western blot analysis of extracts of various cell lines, using Myelin Basic protein antibody (CAB1664) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunohistochemistry of paraffin-embedded mouse brain using Myelin Basic protein Rabbit pAb (CAB1664) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat brain using Myelin Basic protein Rabbit pAb (CAB1664) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human brain using Myelin Basic protein Rabbit pAb (CAB1664) at dilution of 1:100 (40x lens). |
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