Anti-MX1 항체 (CAB1780)

Antibody Name:	Anti-MX1 Antibody
Antibody SKU:	CAB1780
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 368-662 of human MX1 (NP_001171517.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	Raji, HeLa, A-549, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 368-662 of human MX1 (NP_001171517.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ENEK MFFL IDKV NAFN QDIT ALMQ GEET VGEE DIRL FTRL RHEF HKWS TIIE NNFQ EGHK ILSR KIQK FENQ YRGR ELPG FVNY RTFE TIVK QQIK ALEE PAVD MLHT VTDM VRLA FTDV SIKN FEEF FNLH RTAK SKIE DIRA EQER EGEK LIRL HFQM EQIV YCQD QVYR GALQ KVRE KELE EEKK KKSW DFGA FQSS SATD SSME EIFQ HLMA YHQE ASKR ISSH IPLI IQFF MLQT YGQQ LQKA MLQL LQDK DTYS WLLK ERSD TSDK RKFL KERL ARLT QARR RLAQ FPG
Gene ID:	4599
Uniprot:	P20591
Cellular Location:	Cytoplasm, Cytoplasmic side, Endoplasmic reticulum membrane, Nucleus, Peripheral membrane protein, perinuclear region
Calculated MW:	55kDa/75kDa
Observed MW:	78kDa

Synonyms:

MX1, IFI-78K, IFI78, MX, MxA

Background:

This gene encodes a guanosine triphosphate (GTP)-metabolizing protein that participates in the cellular antiviral response. The encoded protein is induced by type I and type II interferons and antagonizes the replication process of several different RNA and DNA viruses. There is a related gene located adjacent to this gene on chromosome 21, and there are multiple pseudogenes located in a cluster on chromosome 4. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	MX1: Dynamin-like GTPase acting as a cell-autonomous host restriction factor against many viral pathogens. Shows activity against influenza viruses, rhabdovirus VSV, bunyavirus LACV, Thogoto virus, measles virus, Hantaan virus, Coxsackie virus CVB, Rift Valley fever virus RVFV, hepatitis B virus HBV, and Crimean- Congo hemorrhagic fever virus CCHFV. Belongs to the dynamin family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Hydrolase; Apoptosis Chromosomal Location of Human Ortholog: 21q22.3 Cellular Component: endoplasmic reticulum membrane; nuclear membrane; perinuclear region of cytoplasm; cytoplasm; cytosol Molecular Function:GTPase activity; protein binding; GTP binding Biological Process: negative regulation of viral genome replication; apoptosis; metabolic process; cytokine and chemokine mediated signaling pathway; response to virus; innate immune response; defense response; signal transduction; defense response to virus
NCBI Summary:	This gene encodes a guanosine triphosphate (GTP)-metabolizing protein that participates in the cellular antiviral response. The encoded protein is induced by type I and type II interferons and antagonizes the replication process of several different RNA and DNA viruses. There is a related gene located adjacent to this gene on chromosome 21, and there are multiple pseudogenes located in a cluster on chromosome 4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]
UniProt Code:	P20591
NCBI GenInfo Identifier:	251757499
NCBI Gene ID:	4599
NCBI Accession:	P20591.4
UniProt Related Accession:	P20591
Molecular Weight:
NCBI Full Name:	Interferon-induced GTP-binding protein Mx1
NCBI Synonym Full Names:	MX dynamin like GTPase 1
NCBI Official Symbol:	MX1
NCBI Official Synonym Symbols:	MX; MxA; IFI78; IFI-78K
NCBI Protein Information:	interferon-induced GTP-binding protein Mx1
UniProt Protein Name:	Interferon-induced GTP-binding protein Mx1
UniProt Synonym Protein Names:	Interferon-induced protein p78; IFI-78K; Interferon-regulated resistance GTP-binding protein MxA; Myxoma resistance protein 1; Myxovirus resistance protein 1Interferon-induced GTP-binding protein Mx1, N-terminally processed
Protein Family:	Interferon-induced GTP-binding protein
UniProt Gene Name:	MX1
UniProt Entry Name:	MX1_HUMAN

Western blot analysis of extracts of various cell lines, using MX1 antibody (CAB1780) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.

Antibodies

MX1 Antibody (PACO10666)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)