Anti-MTNR1A 항체 (CAB13030)

Antibody Name:	Anti-MTNR1A Antibody
Antibody SKU:	CAB13030
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 281-350 of human MTNR1A (NP_005949.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, Mouse small intestine, Rat small intestine, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 281-350 of human MTNR1A (NP_005949.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	YYMA YFNS CLNA IIYG LLNQ NFRK EYRR IIVS LCTA RVFF VDSS NDVA DRVK WKPS PLMT NNNV VKVD SV
Gene ID:	4543
Uniprot:	P48039
Cellular Location:	Cell membrane, Multi-pass membrane protein
Calculated MW:	39kDa
Observed MW:	39kDa

Synonyms:

MTNR1A, MEL-1A-R, MT1

Background:

This gene encodes one of two high affinity forms of a receptor for melatonin, the primary hormone secreted by the pineal gland. This receptor is a G-protein coupled, 7-transmembrane receptor that is responsible for melatonin effects on mammalian circadian rhythm and reproductive alterations affected by day length. The receptor is an integral membrane protein that is readily detectable and localized to two specific regions of the brain. The hypothalamic suprachiasmatic nucleus appears to be involved in circadian rhythm while the hypophysial pars tuberalis may be responsible for the reproductive effects of melatonin.

UniProt Protein Function:	MTNR1A: High affinity receptor for melatonin. Likely to mediates the reproductive and circadian actions of melatonin. The activity of this receptor is mediated by pertussis toxin sensitive G proteins that inhibit adenylate cyclase activity. Belongs to the G-protein coupled receptor 1 family.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Membrane protein, multi-pass; GPCR, family 1; Membrane protein, integral; Receptor, GPCR Chromosomal Location of Human Ortholog: 4q35.1 Cellular Component: integral to plasma membrane; plasma membrane; receptor complex Molecular Function:hormone binding; protein binding Biological Process: cell surface receptor linked signal transduction; G-protein signaling, coupled to cyclic nucleotide second messenger; mating behavior; positive regulation of cGMP biosynthetic process
NCBI Summary:	This gene encodes one of two high affinity forms of a receptor for melatonin, the primary hormone secreted by the pineal gland. This receptor is a G-protein coupled, 7-transmembrane receptor that is responsible for melatonin effects on mammalian circadian rhythm and reproductive alterations affected by day length. The receptor is an integral membrane protein that is readily detectable and localized to two specific regions of the brain. The hypothalamic suprachiasmatic nucleus appears to be involved in circadian rhythm while the hypophysial pars tuberalis may be responsible for the reproductive effects of melatonin. [provided by RefSeq, Jul 2008]
UniProt Code:	P48039
NCBI GenInfo Identifier:	1346544
NCBI Gene ID:	4543
NCBI Accession:	P48039.1
UniProt Secondary Accession:	P48039,A0AVC5, B0M0L2,
UniProt Related Accession:	P48039
Molecular Weight:	39,375 Da
NCBI Full Name:	Melatonin receptor type 1A
NCBI Synonym Full Names:	melatonin receptor 1A
NCBI Official Symbol:	MTNR1A
NCBI Official Synonym Symbols:	MT1; MEL-1A-R
NCBI Protein Information:	melatonin receptor type 1A
UniProt Protein Name:	Melatonin receptor type 1A
Protein Family:	Melatonin receptor
UniProt Gene Name:	MTNR1A
UniProt Entry Name:	MTR1A_HUMAN

	Western blot analysis of extracts of various cell lines, using MTNR1A antibody (CAB13030) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded Rat brain using MTNR1A antibody (CAB13030) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Mouse brain using MTNR1A antibody (CAB13030) at dilution of 1:100 (40x lens).

Antibodies

MTNR1A Antibody (PACO04363)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)