Anti-MTHFD2 항체 (CAB10386)

Antibody Name:	Anti-MTHFD2 Antibody
Antibody SKU:	CAB10386
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 40-250 of human MTHFD2 (NP_006627.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	LO2, U-87MG, HT-29, 293T, DU145, Mouse pancreas

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 40-250 of human MTHFD2 (NP_006627.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ISGR KLAQ QIKQ EVRQ EVEE WVAS GNKR PHLS VILV GENP ASHS YVLN KTRA AAVV GINS ETIM KPAS ISEE ELLN LINK LNND DNVD GLLV QLPL PEHI DERR ICNA VSPD KDVD GFHV INVG RMCL DQYS MLPA TPWG VWEI IKRT GIPT LGKN VVVA GRSK NVGM PIAM LLHT DGAH ERPG GDAT VTIS HRYT PKEQ LKKH TILA DIV
Gene ID:	10797
Uniprot:	P13995
Cellular Location:	Mitochondrion
Calculated MW:	26kDa/37kDa
Observed MW:	38kDa

Synonyms:

MTHFD2, NMDMC

Background:

This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7.

UniProt Protein Function:	MTHFD2: a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7. [provided by RefSeq, Mar 2009]
UniProt Protein Details:	Protein type:Carbohydrate Metabolism - glyoxylate and dicarboxylate; EC 3.5.4.9; Hydrolase; Oxidoreductase; EC 1.5.1.15; Mitochondrial; Cofactor and Vitamin Metabolism - one carbon pool by folate Chromosomal Location of Human Ortholog: 2p13.1 Cellular Component: cytosol; extracellular space; mitochondrial matrix; mitochondrion Molecular Function:formate-tetrahydrofolate ligase activity; magnesium ion binding; methenyltetrahydrofolate cyclohydrolase activity; methylenetetrahydrofolate dehydrogenase (NAD+) activity; methylenetetrahydrofolate dehydrogenase (NADP+) activity; phosphate binding Biological Process: folic acid metabolic process; one-carbon compound metabolic process; tetrahydrofolate metabolic process
NCBI Summary:	This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7. [provided by RefSeq, Mar 2009]
UniProt Code:	P13995
NCBI GenInfo Identifier:	115311607
NCBI Gene ID:	10797
NCBI Accession:	P13995.2
UniProt Secondary Accession:	P13995,Q53G90, Q53GV5, Q53S36, Q7Z650,
UniProt Related Accession:	P13995
Molecular Weight:	26,849 Da
NCBI Full Name:	Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial
NCBI Synonym Full Names:	methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase
NCBI Official Symbol:	MTHFD2
NCBI Official Synonym Symbols:	NMDMC
NCBI Protein Information:	bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial
UniProt Protein Name:	Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial
Protein Family:	Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase
UniProt Gene Name:	MTHFD2
UniProt Entry Name:	MTDC_HUMAN

	Western blot analysis of extracts of various cell lines, using MTHFD2 antibody (CAB10386) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded human mammary cancer using MTHFD2 antibody (CAB10386) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human liver cancer using MTHFD2 antibody (CAB10386) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat kidney using MTHFD2 antibody (CAB10386) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using MTHFD2 antibody (CAB10386) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
MTHFD2 Antibody (PACO10640)	Human MTHFD2 (Methylenetetrahydrofolate dehydrogenase 2) ELISA Kit (HUFI07564)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)