Anti-MTHFD1L Antibody (CAB7969)
- SKU:
- CAB7969
- Product type:
- Antibody
- Application:
- WB
- Application:
- IF
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | MTHFD1L Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB7969 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 759-978 of human MTHFD1L (NP_056255.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-549, placenta, Mouse brain, Mouse heart, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 759-978 of human MTHFD1L (NP_056255.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | GVPL KKEY TEEN IQLV ADGC CNLQ KQIQ ITQL FGVP VVVA LNVF KTDT RAEI DLVC ELAK RAGA FDAV PCYH WSVG GKGS VDLA RAVR EAAS KRSR FQFL YDVQ VPIV DKIR TIAQ AVYG AKDI ELSP EAQA KIDR YTQQ GFGN LPIC MAKT HLSL SHQP DKKG VPRD FILP ISDV RASI GAGF IYPL VGTM STMP GLPT RPCF YDID LDTE TEQV KGLF |
| Gene ID: | 25902 |
| Uniprot: | Q6UB35 |
| Cellular Location: | Mitochondrion |
| Calculated MW: | 29kDa/105kDa |
| Observed MW: | 106kDa |
| Synonyms: | MTHFD1L, FTHFSDC1, MTC1THFS, dJ292B18.2 |
| Background: | The protein encoded by this gene is involved in the synthesis of tetrahydrofolate (THF) in the mitochondrion. THF is important in the de novo synthesis of purines and thymidylate and in the regeneration of methionine from homocysteine. Several transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | MTHFD1L: May provide the missing metabolic reaction required to link the mitochondria and the cytoplasm in the mammalian model of one-carbon folate metabolism in embryonic an transformed cells complementing thus the enzymatic activities of MTHFD2. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Carbohydrate Metabolism - glyoxylate and dicarboxylate; EC 6.3.4.3; Mitochondrial; Cofactor and Vitamin Metabolism - one carbon pool by folate; Ligase Chromosomal Location of Human Ortholog: 6q25.1 Cellular Component: membrane; mitochondrion; cytosol Molecular Function:methylenetetrahydrofolate dehydrogenase (NADP+) activity; protein homodimerization activity; formate-tetrahydrofolate ligase activity; methenyltetrahydrofolate cyclohydrolase activity; ATP binding Biological Process: folic acid and derivative metabolic process; tetrahydrofolate metabolic process; folic acid and derivative biosynthetic process; formate metabolic process; one-carbon compound metabolic process |
| NCBI Summary: | The protein encoded by this gene is involved in the synthesis of tetrahydrofolate (THF) in the mitochondrion. THF is important in the de novo synthesis of purines and thymidylate and in the regeneration of methionine from homocysteine. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jun 2011] |
| UniProt Code: | Q6UB35 |
| NCBI GenInfo Identifier: | 74749360 |
| NCBI Gene ID: | 25902 |
| NCBI Accession: | Q6UB35.1 |
| UniProt Related Accession: | Q6UB35 |
| Molecular Weight: | 106kDa |
| NCBI Full Name: | Monofunctional C1-tetrahydrofolate synthase, mitochondrial |
| NCBI Synonym Full Names: | methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like |
| NCBI Official Symbol: | MTHFD1L |
| NCBI Official Synonym Symbols: | FTHFSDC1; MTC1THFS; dJ292B18.2 |
| NCBI Protein Information: | monofunctional C1-tetrahydrofolate synthase, mitochondrial |
| UniProt Protein Name: | Monofunctional C1-tetrahydrofolate synthase, mitochondrial |
| UniProt Synonym Protein Names: | Formyltetrahydrofolate synthetase |
| Protein Family: | Monofunctional C1-tetrahydrofolate synthase |
| UniProt Gene Name: | MTHFD1L |
| UniProt Entry Name: | C1TM_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MTHFD1L antibody (CAB7969) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat brain using MTHFD1L Rabbit pAb (CAB7969) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse testis using MTHFD1L Rabbit pAb (CAB7969) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse spinal cord using MTHFD1L Rabbit pAb (CAB7969) at dilution of 1:200 (40x lens). |
 | Immunofluorescence analysis of A549 cells using MTHFD1L antibody (CAB7969). Blue: DAPI for nuclear staining. |
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