Anti-MTDH Antibody (CAB5887)
- SKU:
- CAB5887
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-MTDH Antibody |
| Antibody SKU: | CAB5887 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human MTDH (NP_848927.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 22Rv1 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human MTDH (NP_848927.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAAR SWQD ELAQ QAEE GSAR LREM LSVG LGFL RTEL GLDL GLEP KRYP GWVI LVGT GALG LLLL FLLG YGWA AACA GARK KRRS PPRK REEA AAVP AAAP DDLA LLKN LRSE EQKK KNRK KLSE KPKP NGRT VEVA EGEA VRTP QSVT AKQP PEID KKNE KSKK NKKK SKSD AKAV QNSS RHDG KEVD EGAW ETKI SHRE KRQQ RKRD KVLT DSGS LDST IPGI ENTI TVTT EQLT TASF PVGS KKNK GDSH LNVQ VSNF |
| Gene ID: | 92140 |
| Uniprot: | Q86UE4 |
| Cellular Location: | Cell junction, Cytoplasm, Endoplasmic reticulum membrane, Nucleus, Nucleus membrane, Single-pass membrane protein, nucleolus, perinuclear region, tight junction |
| Calculated MW: | 63kDa |
| Observed MW: | 64kDa |
| Synonyms: | MTDH, 3D3, AEG-1, AEG1, LYRIC, LYRIC/3D3 |
| Background: |
| UniProt Protein Function: | Lyric: a protein of unknown function. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Nucleolus; Cell adhesion Chromosomal Location of Human Ortholog: 8q22.1 Cellular Component: nuclear body; intercellular canaliculus; endoplasmic reticulum membrane; nuclear membrane; tight junction; endoplasmic reticulum; perinuclear region of cytoplasm; apical plasma membrane; cytoplasm; nucleolus; integral to membrane; nucleus Molecular Function:NF-kappaB binding; protein binding; double-stranded RNA binding; transcription coactivator activity Biological Process: positive regulation of protein kinase B signaling cascade; positive regulation of angiogenesis; positive regulation of I-kappaB kinase/NF-kappaB cascade; lipopolysaccharide-mediated signaling pathway; negative regulation of transcription from RNA polymerase II promoter; positive regulation of autophagy; negative regulation of apoptosis; activation of NF-kappaB transcription factor |
| UniProt Code: | Q86UE4 |
| NCBI GenInfo Identifier: | 28277147 |
| NCBI Gene ID: | 92140 |
| NCBI Accession: | AAH45642.1 |
| UniProt Secondary Accession: | Q86UE4,Q05DH2, Q52QU9, Q6PK07, Q8TCX3, |
| Molecular Weight: | 63,837 Da |
| NCBI Full Name: | Metadherin |
| NCBI Synonym Full Names: | metadherin |
| NCBI Official Symbol: | MTDH |
| NCBI Official Synonym Symbols: | 3D3; AEG1; AEG-1; LYRIC; LYRIC/3D3 |
| NCBI Protein Information: | protein LYRIC |
| UniProt Protein Name: | Protein LYRIC |
| UniProt Synonym Protein Names: | 3D3/LYRIC; Astrocyte elevated gene-1 protein; AEG-1; Lysine-rich CEACAM1 co-isolated protein; Metadherin; Metastasis adhesion protein |
| Protein Family: | Protein LYRIC |
| UniProt Gene Name: | MTDH |
| UniProt Entry Name: | LYRIC_HUMAN |
 | Western blot analysis of extracts of 22Rv1 cells, using MTDH antibody (CAB5887) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat brain using MTDH antibody (CAB5887) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human gastric using MTDH antibody (CAB5887) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using MTDH antibody (CAB5887) at dilution of 1:200 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using MTDH antibody (CAB5887) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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