Anti-MTAP Antibody (CAB1049)
- SKU:
- CAB1049
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-MTAP Antibody |
| Antibody SKU: | CAB1049 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-283 of human MTAP (NP_002442.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 22Rv1, 293T, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-283 of human MTAP (NP_002442.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MASG TTTT AVKI GIIG GTGL DDPE ILEG RTEK YVDT PFGK PSDA LILG KIKN VDCV LLAR HGRQ HTIM PSKV NYQA NIWA LKEE GCTH VIVT TACG SLRE EIQP GDIV IIDQ FIDR TTMR PQSF YDGS HSCA RGVC HIPM AEPF CPKT REVL IETA KKLG LRCH SKGT MVTI EGPR FSSR AESF MFRT WGAD VINM TTVP EVVL AKEA GICY ASIA MATD YDCW KEHE EAVS VDRV LKTL KENA NKAK SLLL TTIP QIGS TEWS ETLH NLKN MAQF SVLL PRH |
| Gene ID: | 4507 |
| Uniprot: | Q13126 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 26kDa/30kDa/31kDa/32kDa/33kDa/36kDa/38kDa |
| Observed MW: | 31kDa |
| Synonyms: | MTAP, BDMF, DMSFH, DMSMFH, HEL-249, LGMBF, MSAP, c86fus |
| Background: | This gene encodes an enzyme that plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine. The encoded enzyme is deficient in many cancers because this gene and the tumor suppressor p16 gene are co-deleted. Multiple alternatively spliced transcript variants have been described for this gene, but their full-length natures remain unknown. |
| UniProt Protein Function: | MTAP: Catalyzes the reversible phosphorylation of S-methyl-5'- thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S- adenosylmethionine. Has broad substrate specificity with 6- aminopurine nucleosides as preferred substrates. Homotrimer. Ubiquitously expressed. Inhibited by 5'-methylthiotubercin and 5'- chloroformycin. Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily. |
| UniProt Protein Details: | Protein type:Amino Acid Metabolism - cysteine and methionine; EC 2.4.2.28; Phosphorylase; Transferase Chromosomal Location of Human Ortholog: 9p21.3 Cellular Component: cytoplasm; cytosol Molecular Function:phosphorylase activity; protein binding; S-methyl-5-thioadenosine phosphorylase activity Biological Process: methionine salvage; nicotinamide riboside catabolic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; purine salvage |
| NCBI Summary: | This gene encodes an enzyme that plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine. The encoded enzyme is deficient in many cancers because this gene and the tumor suppressor p16 gene are co-deleted. Multiple alternatively spliced transcript variants have been described for this gene, but their full-length natures remain unknown. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q13126 |
| NCBI GenInfo Identifier: | 143811423 |
| NCBI Gene ID: | 4507 |
| NCBI Accession: | Q13126.2 |
| UniProt Secondary Accession: | Q13126,Q5T3P3, Q9H010, I2G7M5, I2G7M6, I2G7M7, I2G7M8 I2G7M9, I2G7N0, |
| UniProt Related Accession: | Q13126 |
| Molecular Weight: | 26,278 Da |
| NCBI Full Name: | S-methyl-5'-thioadenosine phosphorylase |
| NCBI Synonym Full Names: | methylthioadenosine phosphorylase |
| NCBI Official Symbol: | MTAP |
| NCBI Official Synonym Symbols: | BDMF; MSAP; DMSFH; LGMBF; DMSMFH; c86fus; HEL-249 |
| NCBI Protein Information: | S-methyl-5'-thioadenosine phosphorylase |
| UniProt Protein Name: | S-methyl-5'-thioadenosine phosphorylase |
| UniProt Synonym Protein Names: | 5'-methylthioadenosine phosphorylase |
| UniProt Gene Name: | MTAP |
 | Western blot analysis of extracts of various cell lines, using MTAP antibody (CAB1049) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat brain using MTAP antibody (CAB1049) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse liver using MTAP antibody (CAB1049) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of A-549 cells using MTAP antibody (CAB1049). Blue: DAPI for nuclear staining. |
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