Anti-MRPS18B Antibody (CAB12085)
- SKU:
- CAB12085
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-MRPS18B Antibody |
| Antibody SKU: | CAB12085 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-258 of human MRPS18B (NP_054765.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, A-431, Mouse kidney, Mouse brain, Rat heart, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-258 of human MRPS18B (NP_054765.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAAS VLNT VLRR LPML SLFR GSHR VQVP LQTL CTKA PSEE DSLS SVPI SPYK DEPW KYLE SEEY QERY GSRP VWAD YRRN HKGG VPPQ RTRK TCIR RNKV VGNP CPIC RDHK LHVD FRNV KLLE QFVC AHTG IIFY APYT GVCV KQHK RLTQ AIQK ARDH GLLI YHIP QVEP RDLD FSTS HGAV SATP PAPT LVSG DPWY PWYN WKQP PERE LSRL RRLY QGHL QEES GPPP ESMP KMPP RTPA EASS TGQT GPQS AL |
| Gene ID: | 28973 |
| Uniprot: | Q9Y676 |
| Cellular Location: | Mitochondrion |
| Calculated MW: | 29kDa |
| Observed MW: | 35kDa |
| Synonyms: | MRPS18B, C6orf14, HSPC183, HumanS18a, MRP-S18-2, MRPS18-2, PTD017, S18amt |
| Background: | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S18P family. The encoded protein is one of three that has significant sequence similarity to bacterial S18 proteins. The primary sequences of the three human mitochondrial S18 proteins are no more closely related to each other than they are to the prokaryotic S18 proteins. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q. |
| UniProt Protein Function: | MRPS18B: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S18P family. The encoded protein is one of three that has significant sequence similarity to bacterial S18 proteins. The primary sequences of the three human mitochondrial S18 proteins are no more closely related to each other than they are to the prokaryotic S18 proteins. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q. [provided by RefSeq, Jul 2008] |
| UniProt Protein Details: | Protein type:Mitochondrial; Ribosomal; Translation Chromosomal Location of Human Ortholog: 6p21.33 Cellular Component: cell junction; mitochondrial inner membrane; mitochondrial small ribosomal subunit; mitochondrion; nucleoplasm Molecular Function:structural constituent of ribosome Biological Process: mitochondrial translation |
| NCBI Summary: | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S18P family. The encoded protein is one of three that has significant sequence similarity to bacterial S18 proteins. The primary sequences of the three human mitochondrial S18 proteins are no more closely related to each other than they are to the prokaryotic S18 proteins. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9Y676 |
| NCBI GenInfo Identifier: | 24212203 |
| NCBI Gene ID: | 28973 |
| NCBI Accession: | Q9Y676.1 |
| UniProt Secondary Accession: | Q9Y676,Q659G4, Q9BS27, A6NDQ0, |
| UniProt Related Accession: | Q9Y676 |
| Molecular Weight: | 29kDa |
| NCBI Full Name: | 28S ribosomal protein S18b, mitochondrial |
| NCBI Synonym Full Names: | mitochondrial ribosomal protein S18B |
| NCBI Official Symbol: | MRPS18B |
| NCBI Official Synonym Symbols: | PTD017; S18amt; C6orf14; HSPC183; MRPS18-2; HumanS18a; MRP-S18-2 |
| NCBI Protein Information: | 28S ribosomal protein S18b, mitochondrial |
| UniProt Protein Name: | 28S ribosomal protein S18b, mitochondrial |
| UniProt Synonym Protein Names: | 28S ribosomal protein S18-2, mitochondrial; MRP-S18-2; Mitochondrial small ribosomal subunit protein bS18b |
| Protein Family: | 28S ribosomal protein |
| UniProt Gene Name: | MRPS18B |
 | Western blot analysis of extracts of various cell lines, using MRPS18B antibody (CAB12085) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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