Anti-MRPL32 Antibody (CAB10016)
- SKU:
- CAB10016
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-MRPL32 Antibody |
| Antibody SKU: | CAB10016 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 19-188 of human MRPL32 (NP_114109.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Jurkat, Raji, MCF7, HepG2, A-549, Mouse heart, Mouse kidney, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 19-188 of human MRPL32 (NP_114109.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VLRN YWER LLRK LPQS RPGF PSPP WGPA LAVQ GPAM FTEP ANDT SGSK ENSS LLDS IFWM AAPK NRRT IEVN RCRR RNPQ KLIK VKNN IDVC PECG HLKQ KHVL CAYC YEKV CKET AEIR RQIG KQEG GPFK APTI ETVV LYTG ETPS EQDQ GKRI IERD RKRP SWFT QN |
| Gene ID: | 64983 |
| Uniprot: | Q9BYC8 |
| Cellular Location: | Mitochondrion |
| Calculated MW: | 21kDa |
| Observed MW: | 14kDa |
| Synonyms: | MRPL32, HSPC283, L32mt, MRP-L32, bMRP-59b |
| Background: | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L32 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome Xp. |
| UniProt Protein Function: | MRPL32: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L32 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome Xp. [provided by RefSeq, Jul 2008] |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 7p14 Cellular Component: mitochondrial inner membrane; mitochondrial ribosome; large ribosomal subunit Molecular Function:structural constituent of ribosome Biological Process: mitochondrial translation; translation; organelle organization and biogenesis |
| NCBI Summary: | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L32 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome Xp. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9BYC8 |
| NCBI GenInfo Identifier: | 13994261 |
| NCBI Gene ID: | 64983 |
| NCBI Accession: | NP_114109.1 |
| UniProt Secondary Accession: | Q9BYC8,Q96Q68, Q9P098, |
| UniProt Related Accession: | Q9BYC8 |
| Molecular Weight: | 21,405 Da |
| NCBI Full Name: | 39S ribosomal protein L32, mitochondrial |
| NCBI Synonym Full Names: | mitochondrial ribosomal protein L32 |
| NCBI Official Symbol: | MRPL32 |
| NCBI Official Synonym Symbols: | L32mt; HSPC283; MRP-L32; bMRP-59b |
| NCBI Protein Information: | 39S ribosomal protein L32, mitochondrial |
| UniProt Protein Name: | 39S ribosomal protein L32, mitochondrial |
| Protein Family: | 39S ribosomal protein |
| UniProt Gene Name: | MRPL32 |
| UniProt Entry Name: | RM32_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MRPL32 antibody (CAB10016) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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