Anti-MRPL23 Antibody (CAB16424)
- SKU:
- CAB16424
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-MRPL23 Antibody |
| Antibody SKU: | CAB16424 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-153 of human MRPL23 (NP_066957.3). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, U-251MG, A-549, Mouse liver, Mouse lung, Mouse brain, Rat heart, Rat liver, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-153 of human MRPL23 (NP_066957.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MARN VVYP LYRL GGPQ LRVF RTNF FIQL VRPG VAQP EDTV QFRI PMEM TRVD LRNY LEGI YNVP VAAV RTRV QHGS NKRR DHRN VRIK KPDY KVAY VQLA HGQT FTFP DLFP EKDE SPEG SAAD DLYS MLEE ERQQ RQSS DPRR GGVP SWFG L |
| Gene ID: | 6150 |
| Uniprot: | Q16540 |
| Cellular Location: | Mitochondrion |
| Calculated MW: | 17kDa |
| Observed MW: | 15kDa |
| Synonyms: | MRPL23, L23MRP, RPL23, RPL23L |
| Background: | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. The gene is biallelically expressed, despite its location within a region of imprinted genes on chromosome 11. |
| UniProt Protein Function: | MRPL23: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. The gene is biallelically expressed, despite its location within a region of imprinted genes on chromosome 11. [provided by RefSeq, Jul 2008] |
| UniProt Protein Details: | Protein type:Mitochondrial; Ribosomal; RNA-binding Chromosomal Location of Human Ortholog: 11p15.5 Cellular Component: mitochondrion; mitochondrial inner membrane; nucleolus; mitochondrial large ribosomal subunit Molecular Function:structural constituent of ribosome; RNA binding; nucleotide binding Biological Process: mitochondrial translation; translation; organelle organization and biogenesis |
| UniProt Code: | Q16540 |
| NCBI GenInfo Identifier: | |
| NCBI Gene ID: | 1433 |
| NCBI Accession: | NP_001306.2 |
| UniProt Secondary Accession: | Q16540,Q96Q71, A8MT29, |
| Molecular Weight: | 17,781 Da |
| NCBI Full Name: | |
| NCBI Synonym Full Names: | choreoathetosis/spasticity, episodic (paroxysmal choreoathetosis/spasticity) |
| NCBI Official Symbol: | CSE |
| NCBI Official Synonym Symbols: | DYT9 |
| UniProt Protein Name: | 39S ribosomal protein L23, mitochondrial |
| UniProt Synonym Protein Names: | L23 mitochondrial-related protein; Ribosomal protein L23-like |
| Protein Family: | Caffeoylshikimate esterase |
| UniProt Gene Name: | MRPL23 |
| UniProt Entry Name: | RM23_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MRPL23 antibody (CAB16424) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry of paraffin-embedded human thyroid cancer using MRPL23 antibody (CAB16424) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human colon carcinoma using MRPL23 antibody (CAB16424) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U-2 OS cells using MRPL23 antibody (CAB16424) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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