Anti-MRPL12 Antibody (CAB8318)
- SKU:
- CAB8318
- Product type:
- Antibody
- Application:
- WB
- Application:
- IHC
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | MRPL12 Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB8318 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-198 of human MRPL12 (NP_002940.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
| Reactivity: | Human |
| Positive Samples: | A-431, 22Rv1, A-549 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-198 of human MRPL12 (NP_002940.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MLPA AARP LWGP CLGL RAAA FRLA RRQV PCVC AVRH MRSS GHQR CEAL AGAP LDNA PKEY PPKI QQLV QDIA SLTL LEIS DLNE LLKK TLKI QDVG LVPM GGVM SGAV PAAA AQEA VEED IPIA KERT HFTV RLTE AKPV DKVK LIKE IKNY IQGI NLVQ AKKL VESL PQEI KANV AKAE AEKI KAAL EAVG GTVV LE |
| Gene ID: | 6182 |
| Uniprot: | P52815 |
| Cellular Location: | Mitochondrion |
| Calculated MW: | 21kDa |
| Observed MW: | 21kDa |
| Synonyms: | MRPL12, 5c5-2, L12mt, MRP-L31/34, MRPL7, MRPL7/L12, RPML12 |
| Background: | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein which forms homodimers. In prokaryotic ribosomes, two L7/L12 dimers and one L10 protein form the L8 protein complex. |
| UniProt Protein Function: | MRPL12: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein which forms homodimers. In prokaryotic ribosomes, two L7/L12 dimers and one L10 protein form the L8 protein complex. [provided by RefSeq, Jul 2008] |
| UniProt Protein Details: | Protein type:Ribosomal; Mitochondrial Chromosomal Location of Human Ortholog: 17q25 Cellular Component: mitochondrion; mitochondrial inner membrane; mitochondrial large ribosomal subunit Molecular Function:protein binding; structural constituent of ribosome; RNA binding Biological Process: mitochondrial translation; positive regulation of transcription, DNA-dependent; organelle organization and biogenesis; transcription from mitochondrial promoter |
| NCBI Summary: | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein which forms homodimers. In prokaryotic ribosomes, two L7/L12 dimers and one L10 protein form the L8 protein complex. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P52815 |
| NCBI GenInfo Identifier: | 27436901 |
| NCBI Gene ID: | 6182 |
| NCBI Accession: | NP_002940.2 |
| UniProt Secondary Accession: | P52815,Q969U0, Q9HCA2, Q9UQJ3, |
| UniProt Related Accession: | P52815 |
| Molecular Weight: | 21,348 Da |
| NCBI Full Name: | 39S ribosomal protein L12, mitochondrial |
| NCBI Synonym Full Names: | mitochondrial ribosomal protein L12 |
| NCBI Official Symbol: | MRPL12 |
| NCBI Official Synonym Symbols: | 5c5-2; L12mt; MRPL7; RPML12; MRPL7/L12; MRP-L31/34 |
| NCBI Protein Information: | 39S ribosomal protein L12, mitochondrial; MRP-L12 |
| UniProt Protein Name: | 39S ribosomal protein L12, mitochondrial |
| UniProt Synonym Protein Names: | 5c5-2 |
| Protein Family: | 39S ribosomal protein |
| UniProt Gene Name: | MRPL12 |
| UniProt Entry Name: | RM12_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MRPL12 antibody (CAB8318) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
 | Immunohistochemistry of paraffin-embedded human esophageal smooth muscle using MRPL12 antibody (CAB8318) at dilution of 1:100 (40x lens). |
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