Anti-Mouse TNFα In Vivo Antibody - Low Endotoxin
- SKU:
- IVMB0007
- Product Type:
- In Vivo Monoclonal Antibody
- Clone:
- TN3-19.12
- Protein:
- Tumor Necrosis Factor-Alpha
- Isotype:
- Armenian Hamster IgG
- Reactivity:
- Mouse
- Synonyms:
- Tumor Necrosis Factor Ligand Superfamily Member 2 (TNFSF2)
- Cachectin
- Differentiation-Inducing Factor (DIF)
- Necrosin
- Cytotoxin
- Research Area:
- Cytokines, Chemokines & Receptors
- Endotoxin Level:
- Low Endotoxin
- Host Species:
- Armenian Hamster
- Applications:
- IP
- Neutralizing
- WB
Frequently bought together:
Description
system_update_altTechnical Datasheet
| Product Name: | Anti-Mouse TNFα In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0007 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | TN3-19.12 |
| Protein: | Tumor Necrosis Factor-Alpha |
| Product Type: | Monoclonal Antibody |
| Synonyms: | Tumor Necrosis Factor Ligand Superfamily Member 2 (TNFSF2), Cachectin, Differentiation-Inducing Factor (DIF), Necrosin, Cytotoxin |
| Isotype: | Armenian Hamster IgG |
| Reactivity: | Mouse |
| Immunogen: | Recombinant mouse TNFα |
| Applications: | IP, N, WB |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | IP, N, WB |
| Recommended Usage: | ELISA Each lot of this antibody is quality control tested by ELISA assay. For use of this antibody as a capture, a concentration range of 2 - 6 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of mouse TNF-α recombinant protein ranging from 500 to 4 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application. |
| Reactivity: | Mouse |
| Host Species: | Armenian Hamster |
| Specificity: | Armenian Hamster Anti-Mouse TNFa (Clone TN3-19.12 ) recognizes Mouse TNFa. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. |
| Antigen Distribution: | Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells |
| Immunogen: | Recombinant mouse TNFα |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
The tumor necrosis factor (TNF-alpha) is a multifaceted polypeptide cytokine known as a mediator of inflammation and immunity.1 It may mediate some of the significant changes in cellular homeostasis which accompany the invasion of the mammalian host by viruses, bacteria, and parasites.2 TNF-α is an acute phase protein which initiates a cascade of cytokines and increases vascular permeability, thereby recruiting macrophage and neutrophils to a site of infection. TNF-α secreted by the macrophage causes blood clotting which serves to contain the infection. TNF-α has been detected in synovial fluid of patients with rheumatoid arthritis. Clone TN3-19.12 antibody can neutralize the bioactivity of natural or recombinant TNF-α
| Technical Datasheet: | View |
| Protein: | Tumor Necrosis Factor-Alpha |
| Research Area: | Immunology, Innate Immunity |
| Armenian Hamster IgG Isotype Control | |
|---|---|
| Clone | PIP |
| Isotype | Armenian Hamster IgG |
| Endotoxin Level | Low Endotoxin |
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