Anti-Mouse PD-1 (CD279) [29F.1A12] In Vivo Antibody - Low Endotoxin

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SKU:
IVMB0031
Product Type:
In Vivo Monoclonal Antibody
Clone:
29F.1A12
Protein:
PD-1
Isotype:
Rat IgG2a kappa
Reactivity:
Mouse
Synonyms:
Programmed Death-1
CD279
Research Area:
Immune Checkpoint & Cancer Biology
Endotoxin Level:
Low Endotoxin
Host Species:
Rat
Applications:
Blocking
CODEX
CyTOF
FC
IHC FF
WB
Frequently bought together:

Description

system_update_altTechnical Datasheet
Product Name:Anti-Mouse PD-1 (CD279) [29F.1A12] In Vivo Antibody - Low Endotoxin
Product Code:IVMB0031
Size:1mg, 5mg, 25mg, 50mg, 100mg
Clone:29F.1A12
Protein:PD-1
Product Type:Monoclonal Antibody
Synonyms:Programmed Death-1, CD279
Isotype:Rat IgG2a κ
Reactivity:Mouse
Immunogen:PD-1 cDNA followed by PD-1-Ig fusion protein
Applications:B, CyTOF®, FC, IHC FF, PhenoCycler®, WB
Formulation:This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:< 1.0 EU/mg as determined by the LAL method
Purity:≥95% monomer by analytical SEC
>95% by SDS Page
Preparation:Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Product CodeProduct NameEndotoxin Level
IVMB0031Anti-Mouse PD-1 (CD279) [29F.1A12] In Vivo Antibody - Low EndotoxinLow
IVMB0032Anti-Mouse PD-1 (CD279) [29F.1A12] In Vivo Antibody - Ultra Low EndotoxinUltra Low
Applications:B, CODEX®, CyTOF®, FC, IHC FF, WB
Reactivity:Mouse
Host Species:Rat
Specificity:Clone 29F.1A12 recognizes an epitope on mouse PD-1.
Antigen Distribution:PD-1 is expressed on a subset of CD4-CD8- thymocytes, and on activated T and B cells.
Immunogen:PD-1 cDNA followed by PD-1-Ig fusion protein
Concentration:≥ 5.0 mg/ml
Endotoxin Level:< 1.0 EU/mg as determined by the LAL method
Purity:≥95% monomer by analytical SEC
>95% by SDS Page
Formulation:This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

PD-1 is a 50-55 kD member of the B7 Ig superfamily. PD-1 is also a member of the extended CD28/CTLA-4 family of T cell regulators and is suspected to play a role in lymphocyte clonal selection and peripheral tolerance. The ligands of PD-1 are PD-L1 and PD-L2, and are also members of the B7 Ig superfamily. PD-1 and its ligands negatively regulate immune responses. PD-L1, or B7-Homolog 1, is a 40 kD type I transmembrane protein that has been reported to costimulate T cell growth and cytokine production. The interaction of PD-1 with its ligand PD-L1 is critical in the inhibition of T cell responses that include T cell proliferation and cytokine production. PD-L1 has increased expression in several cancers. Inhibition of the interaction between PD-1 and PD-L1 can serve as an immune checkpoint blockade by improving T-cell responses In vitro and mediating preclinical antitumor activity. Within the field of checkpoint inhibition, combination therapy using anti-PD1 in conjunction with anti-CTLA4 has significant therapeutic potential for tumor treatments. PD-L2 is a 25 kD type I transmembrane ligand of PD-1. Via PD-1, PD-L2 can serve as a co-inhibitor of T cell functions. Regulation of T cell responses, including enhanced T cell proliferation and cytokine production, can result from mAbs that block the PD-L2 and PD-1 interaction.

Technical Datasheet:View
Protein:PD-1
Function:Lymphocyte clonal selection, peripheral tolerance
Ligand/Receptor:B7-H1 (PD-L1) and B7-DC (PD-L2)
Research Area:Cancer, Immunology, Inhibitory Molecules
Product Thumbnail
Rat IgG2a Isotype Control
Clone 1-1
Isotype Rat IgG2a
Endotoxin Level Low Endotoxin
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