Anti-Mouse Ly-6G/Ly-6C (Gr-1) In Vivo Antibody - Low Endotoxin

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SKU:
IVMB0091
Product Type:
In Vivo Monoclonal Antibody
Clone:
RB6-8C5
Protein:
Ly-6G/Ly-6C
Isotype:
Rat IgG2b
Reactivity:
Mouse
Synonyms:
Gr-1
Research Area:
Immune Checkpoint & Cancer Biology
Innate Lymphoid Cell Depletion
Neutrophil Cell Depletion
Endotoxin Level:
Low Endotoxin
Host Species:
Rat
Applications:
CyTOF
Depletion
IHC FF
IP
WB
Frequently bought together:

Description

system_update_altTechnical Datasheet
Product Name:Anti-Mouse Ly-6G/Ly-6C (Gr-1) In Vivo Antibody - Low Endotoxin
Product Code:IVMB0091
Size:1mg, 5mg, 25mg, 50mg, 100mg
Clone:RB6-8C5
Protein:Ly-6G/Ly-6C
Product Type:Monoclonal Antibody
Synonyms:Gr-1
Isotype:Rat IgG2b
Reactivity:Mouse
Immunogen:Raised against granulocytes of mouse origin
Applications:CyTOF®, Depletion, IHC FF, IP, WB
Formulation:This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:< 1.0 EU/mg as determined by the LAL method
Purity:≥95% monomer by analytical SEC
>95% by SDS Page
Preparation:Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Product CodeProduct NameEndotoxin Level
IVMB0091Anti-Mouse Ly-6G/Ly-6C (Gr-1) In Vivo Antibody - Low EndotoxinLow
IVMB0092Anti-Mouse Ly-6G/Ly-6C (Gr-1) In Vivo Antibody - Ultra Low EndotoxinUltra Low
Applications:CyTOF®, Depletion, IHC FF, IP, WB
Recommended Usage:FC
The suggested concentration for this RB6-8C5 antibody for staining cells in flow cytometry is ≤ 0.06 µg per 106 in a volume of 100 µl or 100 µl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Reactivity:Mouse
Host Species:Rat
Specificity:Rat Anti-Mouse GR-1, as known as anti-mouse Ly-6G/Ly-6C (Clone RB6-8C5) has been used to identify peripheral blood neutrophils and deplete granulocytes in vivo. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution:The Gr-1 antigen is present at various levels correlated with granulocyte differentiation and maturation. The Gr-1 antigen is expressed on other myeloid populations, but not on lymphoid or erythroid cells.1,2
Immunogen:Raised against granulocytes of mouse origin
Concentration:≥ 5.0 mg/ml
Endotoxin Level:< 1.0 EU/mg as determined by the LAL method
Purity:≥95% monomer by analytical SEC
>95% by SDS Page
Formulation:This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Gr-1 is a 21-25 kD protein. This myeloid differentiation antigen is a glycosylphosphatidylinositol-linked protein expressed on granulocytes and macrophages. Clone RB6-8C5 antibody has been shown to inhibit the binding of the clone 1A8 antibody. Clone 1A8 monoclonal antibody reacts specifically with mouse Ly6G with no reported cross-reactivity with Ly6C.

Technical Datasheet:View
Protein:Ly-6G/Ly-6C
Research Area:Immunology, Innate Immunity
Product Thumbnail
Rat IgG2b Isotype Control
Clone 1-2
Isotype Rat IgG2b
Endotoxin Level Low Endotoxin
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