Anti-Mouse Ly-6C [HK1.4] In Vivo Antibody - Low Endotoxin
- SKU:
- IVMB0177
- Product Type:
- In Vivo Monoclonal Antibody
- Clone:
- HK1.4
- Protein:
- Ly-6C
- Isotype:
- Rat IgG2c
- Reactivity:
- Mouse
- Synonyms:
- Lymphocyte antigen 6 complex
- locus C
- Research Area:
- MHC & Immune Cell-Specific
- Endotoxin Level:
- Low Endotoxin
- Host Species:
- Rat
- Applications:
- CODEX
- CyTOF
- IHC FF
- IP
- WB
Frequently bought together:
Description
system_update_altTechnical Datasheet
Product Name: | Anti-Mouse Ly-6C [HK1.4] In Vivo Antibody - Low Endotoxin |
Product Code: | IVMB0177 |
Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
Clone: | HK1.4 |
Protein: | Ly-6C |
Product Type: | Monoclonal Antibody |
Synonyms: | Lymphocyte antigen 6 complex, locus C |
Isotype: | Rat IgG2c |
Reactivity: | Mouse |
Immunogen: | L3 cloned CTL cells |
Applications: | CyTOF®, IHC FF, IP, PhenoCycler®, WB |
Formulation: | This monoclonal antibody is aseptically packaged and formulated in 20 mM Borate, 250 mM Sodium Chloride (NaCl), pH 8.3-8.7 with no carrier protein, potassium, calcium or preservatives added. |
Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
Applications: | CODEX®, CyTOF®, IHC FF, IP, WB |
Reactivity: | Mouse |
Host Species: | Rat |
Specificity: | Rat Anti-Mouse Ly-6C (Clone HK1.4) recognizes an epitope on Mouse Ly-6C. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. |
Antigen Distribution: | The antigen is found on some monocyte/macrophage populations, endothelial cells, thymocytes, NK-cells, T-cell subsets and 40% of bone marrow cells from all mouse strains tested. Clone HK1.4 does not block the binding of clone RB6-8C5 |
Immunogen: | L3 cloned CTL cells |
Concentration: | ≥ 5.0 mg/ml |
Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
Formulation: | This monoclonal antibody is aseptically packaged and formulated in 20 mM Borate, 250 mM Sodium Chloride (NaCl), pH 8.3-8.7 with no carrier protein, potassium, calcium or preservatives added. |
Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.
Technical Datasheet: | View |
Protein: | Ly-6C |
Research Area: | Immunology |