Anti-Mouse IL-10 In Vivo Antibody - Low Endotoxin
- SKU:
- IVMB0084
- Product Type:
- In Vivo Monoclonal Antibody
- Clone:
- JES5-2A5
- Protein:
- IL-10
- Isotype:
- Rat IgG1 kappa
- Synonyms:
- CSIF
- IL10A
- MGC126450
- MGC126451
- TGIF
- Research Area:
- Cytokines, Chemokines & Receptors
- Endotoxin Level:
- Low Endotoxin
- Host Species:
- Rat
- Applications:
- CyTOF
- Neutralizing
- WB
Description
| Product Name: | Anti-Mouse IL-10 In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0084 |
| Size: | 1mg |
| Clone: | JES5-2A5 |
| Protein: | IL-10 |
| Product Type: | Monoclonal Antibody |
| Synonyms: | CSIF, IL10A, MGC126450, MGC126451, TGIF |
| Isotype: | Rat IgG1 κ |
| Reactivity: | Sample Reactivity Species |
| Immunogen: | Purified Recombinant Mouse IL-10 |
| Applications: | CyTOF®, N, WB |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | CyTOF®, N, WB |
| Reactivity: | Sample Reactivity Species |
| Host Species: | Rat |
| Specificity: | Clone JES5-2A5 recognizes mouse IL-10. |
| Antigen Distribution: | OR Cel Targets: T cells, B cells, mast cells, macrophages |
| Immunogen: | Purified Recombinant Mouse IL-10 |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
IL-10 is a homodimeric, anti-inflammatory cytokine of 17-21 kD with various, pleiotropic, effects in immunoregulation and inflammation. It increases antibody production, in addition to enhancing B cell survival and proliferation. IL-10 inhibits both the synthesis of pro-inflammatory cytokines and the actions of NK cells during the immune response to viral infection. Moreover, IL-10 is involved in peripheral T cell tolerance to allergens, autoantigens, transplantation antigens and tumor antigens. IL-10 can also block NF-\uf06bB activity, and is involved in the regulation of the JAK-STAT signaling pathway. In mice, lack of IL-10 has been shown to cause inflammation and pain via COX activation resulting in vascular endothelial and cardiac dysfunctions. Additionally, IL-10 is linked to myokines, a form of cytokine produced in muscle cells that participates in tissue regeneration and repair, maintenance of healthy bodily functioning, and homeostasis in the immune system. Exercise is known to increase circulating levels of IL-10. Hence, it is thought that physical exercise promotes an environment of anti-inflammatory cytokines. Furthermore, knockout studies of IL-10 suggest this cytokine is crucial for counteracting the hyperactive immune response in the intestinal tract. It has been reported that treatment with recombinant IL-10 producing bacteria has been beneficial in patients with Crohn's disease.
| Technical Datasheet: | View |
| Protein: | IL-10 |
| Function: | Sample Function |
| Research Area: | Immunology |
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