Anti-Mouse H-2Kb (MHC Class I) [Y-3] In Vivo Antibody - Ultra Low Endotoxin

(No reviews yet) Write a Review
SKU:
IVMB0010
Product Type:
In Vivo Monoclonal Antibody
Clone:
Y-3
Protein:
MHC Class I
Isotype:
Mouse IgG2b kappa
Reactivity:
Mouse
Synonyms:
H-2K
H-2 class I histocompatibility antigen
K-B alpha chain
H-2K(B)
H-2K(K)
H-2K(Q)
H-2K(R)
H-2K(S)
Beta-2-microglobulin
Research Area:
MHC & Immune Cell-Specific
Endotoxin Level:
Ultra Low Endotoxin
Host Species:
Mouse
Applications:
FA
ICC
IP
WB
Frequently bought together:

Description

system_update_altTechnical Datasheet
Product Name:Anti-Mouse H-2Kb (MHC Class I) [Y-3] In Vivo Antibody - Ultra Low Endotoxin
Product Code:IVMB0010
Size:1mg, 5mg, 25mg, 50mg, 100mg
Clone:Y-3
Protein:MHC Class I
Product Type:Monoclonal Antibody
Synonyms:H-2K, H-2 class I histocompatibility antigen, K-B alpha chain, H-2K(B), H-2K(K), H-2K(Q), H-2K(R), H-2K(S), Beta-2-microglobulin
Isotype:Mouse IgG2b κ
Reactivity:Mouse
Immunogen:Con A stimulated spleen cells from BALB.B mice
Applications:FA, ICC, IP, WB
Formulation:This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:<0.5 EU/mg as determined by the LAL method
Purity:≥98% monomer by analytical SEC
>95% by SDS Page
Preparation:Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Product CodeProduct NameEndotoxin Level
IVMB0009Anti-Mouse H-2Kb (MHC Class I) [Y-3] In Vivo Antibody - Low EndotoxinLow
IVMB0010Anti-Mouse H-2Kb (MHC Class I) [Y-3] In Vivo Antibody - Ultra Low EndotoxinUltra Low
Applications:FA, ICC, IP, WB
Reactivity:Mouse
Host Species:Mouse
Specificity:Clone Y-3 recognizes an epitope on mouse MHC class I H-2K haplotypes b, k, q, r, s, but not d.
Antigen Distribution:H-2K is ubiquitously expressed on nucleated cells.
Immunogen:Con A stimulated spleen cells from BALB.B mice
Concentration:≥ 5.0 mg/ml
Endotoxin Level:<0.5 EU/mg as determined by the LAL method
Purity:≥98% monomer by analytical SEC
>95% by SDS Page
Formulation:This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

H-2K antibody, clone Y-3, recognizes the major histocompatibility complex (MHC) class I H-2K haplotypes b, k, q, r, s, but not d. MHC class I is ubiquitously expressed on the cell surface of nucleated cells and consists of a 45-kDa type I transmembrane glycoprotein (α-chain or heavy chain) and a 12-kDa soluble protein (β2-microglobulin, β2M)1,2. The α-chain consists of three domains (α1, α2, and α3)3. α1 and α2 form the closed antigen-binding groove and bind to 8-10 aa peptides derived from cytosolic antigens4-6. β2M noncovalently associates with α3, which is essential for MHC stability. H-2K plays a critical role in the adaptive immune response by presenting endogenous antigens to cytotoxic CD8 T cells. MHC class I molecules can also present exogenous antigens to CD8 T cells via a process known as cross-presentation7. The T cell receptor (TCR)/CD3 complex of CD8 T cells interacts with peptide-MHC class I, which induces CD8 T cell activation and subsequent cell-killing. CD8 molecules also bind to MHC class I, which helps augment TCR signaling8. In contrast to CD8 T cells, MHC class I is an inhibitory ligand for natural killer (NK) cells, promoting self tolerance9. MHC class I also contributes to the positive selection of CD8 T cells and NK cell specificity10,11.

Technical Datasheet:View
Protein:MHC Class I
Ligand/Receptor:CD3/TCR, CD4
Research Area:Immunology, Innate Immunity
Product Thumbnail
Mouse IgG2b Isotype Control
Clone MPC-11
Isotype Mouse IgG2b
Endotoxin Level Ultra Low Endotoxin
Learn More

0 Reviews

View AllClose

Related Products