Anti-Mouse CD8a (Ly 2) In Vivo Antibody - Ultra Low Endotoxin

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SKU:
IVMB0069
Product Type:
In Vivo Monoclonal Antibody
Clone:
53-6.7
Protein:
CD8a
Isotype:
Rat IgG2a kappa
Reactivity:
Mouse
Synonyms:
T8
Lyt2
Ly-2
Research Area:
CD8+ T Cell Depletion
Endotoxin Level:
Ultra Low Endotoxin
Host Species:
Rat
Applications:
Blocking
CODEX
CyTOF
Depletion
FC
IHC FF
IP
WB
Frequently bought together:

Description

system_update_altTechnical Datasheet
Product Name:Anti-Mouse CD8a (Ly 2) In Vivo Antibody - Ultra Low Endotoxin
Product Code:IVMB0069
Size:1mg, 5mg, 25mg, 50mg, 100mg
Clone:53-6.7
Protein:CD8a
Product Type:Monoclonal Antibody
Synonyms:T8, Lyt2, Ly-2
Isotype:Rat IgG2a κ
Reactivity:Mouse
Immunogen:Mouse thymus or spleen
Applications:B, CyTOF®, Depletion, FC, IHC FF, IP, PhenoCycler®, WB
Formulation:This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:<0.5 EU/mg as determined by the LAL method
Purity:≥98% monomer by analytical SEC
>95% by SDS Page
Preparation:Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Product CodeProduct NameEndotoxin Level
IVMB0068Anti-Mouse CD8a (Ly 2) In Vivo Antibody - Low EndotoxinLow
IVMB0069Anti-Mouse CD8a (Ly 2) In Vivo Antibody - Ultra Low EndotoxinUltra Low
Applications:B, CODEX®, CyTOF®, Depletion, FC, IHC FF, IP, WB
Recommended Usage:FC
The suggested concentration for this 53-6.7 antibody for staining cells in flow cytometry is ≤ 0.25 µg per 106 in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application.
WB
The suggested concentration for this 53-6.7 antibody for use in western blotting is 1-10 µg/ml.
Reactivity:Mouse
Host Species:Rat
Specificity:Clone 53-6.7 recognizes Lyt 2. Clone 53-6.7 competes with clone 5H10-1 for binding to thymocytes.
Antigen Distribution:Lyt 2 is present on the surface of most thymocytes and a subpopulation of mature T-lymphocytes which include most T suppressor/cytotoxic cells.
Immunogen:Mouse thymus or spleen
Concentration:≥ 5.0 mg/ml
Endotoxin Level:<0.5 EU/mg as determined by the LAL method
Purity:≥98% monomer by analytical SEC
>95% by SDS Page
Formulation:This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

CD8 is made up of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8 is part of the Ig superfamily that expresses primarily as CD8a homodimers. CD8a is a 32-34 kD type I glycoprotein that can also form heterodimers with CD8b. CD8 is an antigen co-receptor on T cells that mediates efficient cell to cell interactions within the immune system. CD8 coupled with the T cell receptor on the T lymphocyte recognizes an antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The CD8 co-receptor also plays a role in T cell signaling by interacting with Lck (lymphocyte-specific protein tyrosine kinase) which leads to the activation of transcription factors that affect the expression of certain genes.

Technical Datasheet:View
Protein:CD8a
Ligand/Receptor:MHC class I molecule
Product Thumbnail
Rat IgG2a Isotype Control
Clone 1-1
Isotype Rat IgG2a
Endotoxin Level Ultra Low Endotoxin
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