Anti-Mouse CD62L In Vivo Antibody - Low Endotoxin
- SKU:
- IVMB0064
- Product Type:
- In Vivo Monoclonal Antibody
- Clone:
- MEL-14
- Protein:
- CD62L
- Isotype:
- Rat IgG2a kappa
- Reactivity:
- Mouse
- Synonyms:
- L-selectin
- LECAM-1
- Ly-22
- LAM-1
- MEL-14
- Endotoxin Level:
- Low Endotoxin
- Host Species:
- Rat
- Applications:
- Blocking
- CyTOF
- FA
- FC
- IHC FF
- IP
- WB
Frequently bought together:
Description
system_update_altTechnical Datasheet
| Product Name: | Anti-Mouse CD62L In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0064 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | MEL-14 |
| Protein: | CD62L |
| Product Type: | Monoclonal Antibody |
| Synonyms: | L-selectin, LECAM-1, Ly-22, LAM-1, MEL-14 |
| Isotype: | Rat IgG2a κ |
| Reactivity: | Mouse |
| Immunogen: | C3H/eb mouse B lymphoma 38C-13 |
| Applications: | B, CyTOF®, FA, FC, IHC FF, IP, WB |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | B, CyTOF®, FA, FC, IHC FF, IP, WB |
| Recommended Usage: | FC The suggested concentration for this MEL-14 antibody for staining cells in flow cytometry is ≤ 0.25 µg per 106 in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application. WB The suggested concentration for this MEL-14 antibody for use in western blotting is 1-10 µg/ml. |
| Reactivity: | Mouse |
| Host Species: | Rat |
| Specificity: | Rat Anti-Mouse CD62L (Clone MEL-14) recognizes an epitope on Mouse CD62L. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. |
| Antigen Distribution: | Subsets of B and T cells, monocytes, granulocytes, subset of NK cells |
| Immunogen: | C3H/eb mouse B lymphoma 38C-13 |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
CD62L is a 74-95 kD glycoprotein is a member of the selectin family and is expressed on the majority of B and naïve T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells. Furthermore, CD62L is a cell adhesion molecule that binds to many glycoprotein ligands including CD34, GlyCAM-1, and PSGL-1. Clone MEL-14 is reported as a Nutralization antibody.
| Technical Datasheet: | View |
| Protein: | CD62L |
| Function: | Lymphocyte homing to HEV, rolling on activated endothelium |
| Ligand/Receptor: | CD34, GlyCAM-1, MAdCAM-1 |
| Research Area: | Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity |
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