Anti-Mouse CD28 In Vivo Antibody - Ultra Low Endotoxin

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SKU:
IVMB0057
Product Type:
In Vivo Monoclonal Antibody
Clone:
37.51
Protein:
CD28
Isotype:
Syrian Hamster IgG
Reactivity:
Mouse
Synonyms:
MGC138290
Tp44
Research Area:
Immune Checkpoint & Cancer Biology
Endotoxin Level:
Ultra Low Endotoxin
Host Species:
Syrian Hamster
Applications:
Blocking
Costim
CyTOF
FA
IP
WB
Frequently bought together:

Description

system_update_altTechnical Datasheet
Product Name:Anti-Mouse CD28 In Vivo Antibody - Ultra Low Endotoxin
Product Code:IVMB0057
Size:1mg, 5mg, 25mg, 50mg, 100mg
Clone:37.51
Protein:CD28
Product Type:Monoclonal Antibody
Synonyms:MGC138290, Tp44
Isotype:Syrian Hamster IgG
Reactivity:Mouse
Immunogen:C57BL/6 mouse T-cell lymphoma EL-4
Applications:B, Costim, CyTOF®, FA, IP, WB
Formulation:This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:<0.5 EU/mg as determined by the LAL method
Purity:≥98% monomer by analytical SEC
>95% by SDS Page
Preparation:Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Product CodeProduct NameEndotoxin Level
IVMB0056Anti-Mouse CD28 In Vivo Antibody - Low EndotoxinLow
IVMB0057Anti-Mouse CD28 In Vivo Antibody - Ultra Low EndotoxinUltra Low
Applications:B, Costim, CyTOF®, FA, IP, WB
Recommended Usage:FC
The suggested concentration for this 37.51 antibody for staining cells in flow cytometry is ≤ 1.0 µg per 106 in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application.
WB
The suggested concentration for this 37.51 antibody for use in western blotting is 1-10 µg/ml.
Reactivity:Mouse
Host Species:Syrian Hamster
Specificity:Clone 37.51 recognizes an epitope on mouse CD28.
Antigen Distribution:CD28 is expressed on thymocytes, CD4+, CD8+ peripheral T cells, NK cells.
Immunogen:C57BL/6 mouse T-cell lymphoma EL-4
Concentration:≥ 5.0 mg/ml
Endotoxin Level:<0.5 EU/mg as determined by the LAL method
Purity:≥98% monomer by analytical SEC
>95% by SDS Page
Formulation:This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

CD28 is a 44 kD glycoprotein and a member of the Ig superfamily. In its capacity as a costimulatory receptor, CD28 produces co-stimulatory signals necessary for T cell activation and survival. CD28 is the only B7 receptor constitutively expressed on naive T cells. Without having this CD28/B7 interaction, T cells will fail to respond to their specific antigen when coming in contact with an MHC/antigen complex and thus, are said to be anergic.

Technical Datasheet:View
Protein:CD28
Ligand/Receptor:CD80 (B7-1), CD86 (B7-2)
Research Area:Costimulatory Molecules, Immunology

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