Anti-Mouse CD28 In Vivo Antibody - Low Endotoxin
- SKU:
- IVMB0056
- Product Type:
- In Vivo Monoclonal Antibody
- Clone:
- 37.51
- Protein:
- CD28
- Isotype:
- Syrian Hamster IgG
- Reactivity:
- Mouse
- Synonyms:
- MGC138290
- Tp44
- T44
- Research Area:
- Immune Checkpoint & Cancer Biology
- Endotoxin Level:
- Low Endotoxin
- Host Species:
- Syrian Hamster
- Applications:
- Blocking
- Costim
- CyTOF
- FC
- IHC FF
- IP
- WB
Frequently bought together:
Description
system_update_altTechnical Datasheet
| Product Name: | Anti-Mouse CD28 In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0056 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | 37.51 |
| Protein: | CD28 |
| Product Type: | Monoclonal Antibody |
| Synonyms: | MGC138290, Tp44, T44 |
| Isotype: | Syrian Hamster IgG |
| Reactivity: | Mouse |
| Immunogen: | C57BL/6 mouse T-cell lymphoma EL-4 |
| Applications: | B, Costim, CyTOF®, FC, IHC FF, IP, WB |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | B, Costim, CyTOF®, FC, IHC FF, IP, WB |
| Recommended Usage: | FC The suggested concentration for this 37.51 antibody for staining cells in flow cytometry is ≤ 1.0 µg per 106 in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application. WB The suggested concentration for this 37.51 antibody for use in western blotting is 1-10 µg/ml. |
| Reactivity: | Mouse |
| Host Species: | Syrian Hamster |
| Specificity: | Clone 37.51 recognizes an epitope on mouse CD28. |
| Antigen Distribution: | CD28 is expressed on thymocytes, CD4+, CD8+ peripheral T cells, NK cells. |
| Immunogen: | C57BL/6 mouse T-cell lymphoma EL-4 |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
CD28 is a 44 kD glycoprotein and a member of the Ig superfamily. In its capacity as a costimulatory receptor, CD28 produces co-stimulatory signals necessary for T cell activation and survival. CD28 is the only B7 receptor constitutively expressed on naive T cells. Without having this CD28/B7 interaction, T cells will fail to respond to their specific antigen when coming in contact with an MHC/antigen complex and thus, are said to be anergic.
| Technical Datasheet: | View |
| Protein: | CD28 |
| Function: | Costimulates T and NK cells |
| Ligand/Receptor: | CD80 (B7-1), CD86 (B7-2) |
| Research Area: | Costimulatory Molecules, Immunology |
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