Anti-Mouse CD25 In Vivo Antibody - Low Endotoxin
- SKU:
- IVMB0148
- Product Type:
- In Vivo Monoclonal Antibody
- Clone:
- PC61
- Protein:
- CD25
- Isotype:
- Rat IgG1 lambda
- Reactivity:
- Mouse
- Synonyms:
- IL-2Ralpha
- Ly-43
- p55
- Tac
- Research Area:
- T Reg Cell Depletion
- Endotoxin Level:
- Low Endotoxin
- Host Species:
- Rat
- Applications:
- Blocking
- CODEX
- Depletion
- FA
- FC
- IHC FF
- IP
- WB
Frequently bought together:
Description
system_update_altTechnical Datasheet
| Product Name: | Anti-Mouse CD25 In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0148 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | PC61 |
| Protein: | CD25 |
| Product Type: | Monoclonal Antibody |
| Synonyms: | IL-2Rα, Ly-43, p55, Tac |
| Isotype: | Rat IgG1 Lambda |
| Reactivity: | Mouse |
| Immunogen: | IL-2-dependent cytolytic mouse T-cell clone B6.1 |
| Applications: | B, Depletion, FA, FC, IHC FF, IP, PhenoCycler®, WB |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | B, CODEX®, Depletion, FA, FC, IHC FF, IP, WB |
| Recommended Usage: | FC The suggested concentration for this PC61 antibody for staining cells in flow cytometry is ≤ 1 µg per 106 in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application. WB The suggested concentration for this PC61 antibody for use in western blotting is 1-10 µg/ml. |
| Reactivity: | Mouse |
| Host Species: | Rat |
| Specificity: | Clone PC61 recognizes an epitope on mouse CD25. |
| Antigen Distribution: | CD25 is expressed in activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells. |
| Immunogen: | IL-2-dependent cytolytic mouse T-cell clone B6.1 |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
CD25, a 55 kD type I transmembrane glycoprotein, has been shown to play roles in lymphocyte differentiation, activation, and proliferation. Many resting memory T cells constitutively express IL2RA. It functions as the receptor for HTLV-1, resulting in its expression on neoplastic cells in adult T cell lymphoma/leukemia. CD25 (sIL-2R) has been used to track disease progression. Some additional clinical applications include Chagas disease, a disease characterized by a decline of CD25 expression on immune cells, and Multiple sclerosis, in which treatments with mAbs target CD25.
| Technical Datasheet: | View |
| Protein: | CD25 |
| Function: | Forms high affinity IL-2R with IL-2Rβ (CD122) and IL-2Rγ (CD132) |
| Ligand/Receptor: | IL-2 |
| Research Area: | Immunology |
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