Anti-Mouse CD11c In Vivo Antibody - Low Endotoxin
- SKU:
- IVMB0141
- Product Type:
- In Vivo Monoclonal Antibody
- Clone:
- N418
- Protein:
- CD11c
- Isotype:
- Armenian Hamster IgG
- Reactivity:
- Mouse
- Synonyms:
- alphaX integrin
- integrin alphaX chain
- CR4
- p150
- ITGAX
- Endotoxin Level:
- Low Endotoxin
- Host Species:
- Armenian Hamster
- Applications:
- Blocking
- CODEX
- CyTOF
- IHC FF
- WB
Frequently bought together:
Description
system_update_altTechnical Datasheet
| Product Name: | Anti-Mouse CD11c In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0141 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | N418 |
| Protein: | CD11c |
| Product Type: | Monoclonal Antibody |
| Synonyms: | αX integrin, integrin αX chain, CR4, p150, ITGAX |
| Isotype: | Armenian Hamster IgG |
| Reactivity: | Mouse |
| Immunogen: | Mouse spleen dendritic cells |
| Applications: | B, CyTOF®, IHC FF, PhenoCycler®, WB |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | B, CODEX®, CyTOF®, IHC FF, WB |
| Recommended Usage: | FC The suggested concentration for this N418 antibody for staining cells in flow cytometry is ≤ 1.0 µg per 106 in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application. WB The suggested concentration for this N418 antibody for use in western blotting is 1-10 µg/ml. |
| Reactivity: | Mouse |
| Host Species: | Armenian Hamster |
| Specificity: | Clone N418 recognizes an epitope on mouse CD11C. |
| Antigen Distribution: | CD11c is primarily expressed on dendritic cells, NK cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells. |
| Immunogen: | Mouse spleen dendritic cells |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
LFA-1α (CD11a) and CD18 are the Integrin alpha-L and beta-2 chains respectively that combine to form LFA-1, a glycoprotein and a member of the Integrin family. Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3, ICAM4 and for F11R. LFA-1 participates in the immunological synapses between CD8+ T lymphocytes and antigen-presenting cells. The absence of LFA-1α or β may induce LAD. The antigen contributes to natural killer cell cytotoxicity, and is involved in various immune phenomena such as leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. The CD11b/CD18 antigen is a heterodimeric surface glycoprotein on leukocytes and belongs to the β2 integrin family. CD11b functions as a receptor for C3bi complement, clotting factor X, fibrinogen and ICAM-1. CD11c forms an α/β heterodimeric glycoprotein (CD11c/CD18 complex) which belongs to the β2 integrin family. The complex binds fibrinogen and reportedly serves as a receptor for iC3b and ICAM-1. During inflammatory responses, it mediates cell to cell interaction and is important in both monocyte adhesion and chemotaxis.
| Technical Datasheet: | View |
| Protein: | CD11c |
| Function: | Cellular adhesion |
| Ligand/Receptor: | iC3b, fibrinogen |
| Research Area: | Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers |
| Armenian Hamster IgG Isotype Control | |
|---|---|
| Clone | PIP |
| Isotype | Armenian Hamster IgG |
| Endotoxin Level | Low Endotoxin |
Related Products
Anti-Mouse CD11c In Vivo Antibody - Ultra Low Endotoxin
Anti-Human CD11c In Vivo Antibody - Low Endotoxin
Anti-Human CD11c In Vivo Antibody - Ultra Low Endotoxin
Anti-Mouse CD40 In Vivo Antibody - Low Endotoxin
Anti-Mouse CD22 In Vivo Antibody - Low Endotoxin
Anti-Mouse CD28 In Vivo Antibody - Low Endotoxin
Anti-Mouse IFNß In Vivo Antibody - Low Endotoxin
Anti-Mouse CD115 In Vivo Antibody - Low Endotoxin
