Anti-Mouse CD11b In Vivo Antibody - Low Endotoxin
- SKU:
- IVMB0042
- Product Type:
- In Vivo Monoclonal Antibody
- Clone:
- M1/70
- Protein:
- CD11b
- Isotype:
- Rat IgG2b kappa
- Reactivity:
- Mouse
- Synonyms:
- Mac-1
- Integrin alpham chain
- C3biR
- CR3
- Mo1
- ITGAM
- Endotoxin Level:
- Low Endotoxin
- Host Species:
- Rat
- Applications:
- CODEX
- CyTOF
- FA
- FC
- ICC
- IHC FF
Description
| Product Name: | Anti-Mouse CD11b In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0042 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | M1/70 |
| Protein: | CD11b |
| Product Type: | Monoclonal Antibody |
| Synonyms: | Mac-1, Integrin αm chain, C3biR, CR3, Mo1, ITGAM |
| Isotype: | Rat IgG2b κ |
| Reactivity: | Mouse |
| Immunogen: | B10 mouse spleen cells enriched for T cells |
| Applications: | CyTOF®, FA, FC, ICC, IHC FF, PhenoCycler® |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | CODEX®, CyTOF®, FA, FC, ICC, IHC FF |
| Recommended Usage: | FC The suggested concentration for this M1/70 antibody for staining cells in flow cytometry is ≤ 0.25 µg per 106 in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application. |
| Reactivity: | Mouse |
| Host Species: | Rat |
| Specificity: | Clone M1/70 recognizes the α subunit (CD11b) of the mouse CD11b/CD18 complex. |
| Antigen Distribution: | CD11b is expressed on human peripheral blood lymphocytes, NK lymphocytes, monocytes, granulocytes, a subset of T-cells and macrophages. |
| Immunogen: | B10 mouse spleen cells enriched for T cells |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
LFA-1α (CD11a) and CD18 are the Integrin alpha-L and beta-2 chains respectively that combine to form LFA-1, a glycoprotein and a member of the Integrin family. Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3, ICAM4 and for F11R. LFA-1 participates in the immunological synapses between CD8+ T lymphocytes and antigen-presenting cells. The absence of LFA-1α or β may induce LAD. The antigen contributes to natural killer cell cytotoxicity, and is involved in various immune phenomena such as leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. The CD11b/CD18 antigen is a heterodimeric surface glycoprotein on leukocytes and belongs to the β2 integrin family. CD11b functions as a receptor for C3bi complement, clotting factor X, fibrinogen and ICAM-1. CD11c forms an α/β heterodimeric glycoprotein (CD11c/CD18 complex) which belongs to the β2 integrin family. The complex binds fibrinogen and reportedly serves as a receptor for iC3b and ICAM-1. During inflammatory responses, it mediates cell to cell interaction and is important in both monocyte adhesion and chemotaxis.
| Technical Datasheet: | View |
| Protein: | CD11b |
| Function: | Adhesion, chemotaxis |
| Ligand/Receptor: | ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, fibrinogen |
| Research Area: | Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers |
Meet the team!
Shane Costigan
Territory Manager & Team Lead
Abdul Khadim
Sales Executive
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