Anti-Mouse CD11a [I21/7] In Vivo Antibody - Ultra Low Endotoxin

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SKU:
IVMB0140
Product Type:
In Vivo Monoclonal Antibody
Clone:
I21/7
Protein:
CD11a
Isotype:
Rat IgG2a
Reactivity:
Mouse
Synonyms:
LFA-1alpha
Endotoxin Level:
Ultra Low Endotoxin
Host Species:
Rat
Applications:
FC
Frequently bought together:

Description

system_update_altTechnical Datasheet
Product Name:Anti-Mouse CD11a [I21/7] In Vivo Antibody - Ultra Low Endotoxin
Product Code:IVMB0140
Size:1mg, 5mg, 25mg, 50mg, 100mg
Clone:I21/7
Protein:CD11a
Product Type:Monoclonal Antibody
Synonyms:LFA-1α
Isotype:Rat IgG2a
Reactivity:Mouse
Immunogen:Whole T cells Lymphoma BW5147 cells
Applications:FC
Formulation:This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:<0.5 EU/mg as determined by the LAL method
Purity:≥98% monomer by analytical SEC
>95% by SDS Page
Preparation:Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Product CodeProduct NameEndotoxin Level
IVMB0139Anti-Mouse CD11a [I21/7] In Vivo Antibody - Low EndotoxinLow
IVMB0140Anti-Mouse CD11a [I21/7] In Vivo Antibody - Ultra Low EndotoxinUltra Low
Applications:FC
Recommended Usage:FC
The suggested concentration for clone I21/7 antibody for staining cells in flow cytometry is ≤ 0.5-1.0 µg per 106 in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application.
Reactivity:Mouse
Host Species:Rat
Specificity:Clone I21/7 recognizes an epitope on mouse CD11a.
Antigen Distribution:CD11a is present on thymocytes, blood lymphocytes, bone marrow cells and certain lymphoma and macrophage-like cell lines.
Immunogen:Whole T cells Lymphoma BW5147 cells
Concentration:≥ 5.0 mg/ml
Endotoxin Level:<0.5 EU/mg as determined by the LAL method
Purity:≥98% monomer by analytical SEC
>95% by SDS Page
Formulation:This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

LFA-1α (CD11a) and CD18 are the Integrin alpha-L and beta-2 chains respectively that combine to form LFA-1, a glycoprotein and a member of the Integrin family. Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3, ICAM4 and for F11R. LFA-1 participates in the immunological synapses between CD8+ T lymphocytes and antigen-presenting cells. The absence of LFA-1α or β may induce LAD. The antigen contributes to natural killer cell cytotoxicity, and is involved in various immune phenomena such as leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. The CD11b/CD18 antigen is a heterodimeric surface glycoprotein on leukocytes and belongs to the β2 integrin family. CD11b functions as a receptor for C3bi complement, clotting factor X, fibrinogen and ICAM-1. CD11c forms an α/β heterodimeric glycoprotein (CD11c/CD18 complex) which belongs to the β2 integrin family. The complex binds fibrinogen and reportedly serves as a receptor for iC3b and ICAM-1. During inflammatory responses, it mediates cell to cell interaction and is important in both monocyte adhesion and chemotaxis.

Technical Datasheet:View
Protein:CD11a
Function:Intercellular adhesion
Ligand/Receptor:ICAM-1 (CD54), ICAM-2(CD102), ICAM-3(CD50)
Research Area:Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Signal Transduction
Product Thumbnail
Rat IgG2a Isotype Control
Clone 1-1
Isotype Rat IgG2a
Endotoxin Level Ultra Low Endotoxin
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