Anti-Mouse CD115 In Vivo Antibody - Low Endotoxin
- SKU:
- IVMB0001
- Product Type:
- In Vivo Monoclonal Antibody
- Clone:
- AFS98
- Protein:
- CD115
- Isotype:
- Rat IgG2a kappa
- Reactivity:
- Mouse
- Synonyms:
- Macrophage Colony Stimulating Factor Receptor
- M-CSFR
- CSF-R1
- C-fms
- CSF-1R
- Research Area:
- Innate Lymphoid Cell Depletion
- Macrophage Cell Depletion
- Cytokines, Chemokines & Receptors
- Endotoxin Level:
- Low Endotoxin
- Host Species:
- Rat
- Applications:
- Blocking
- CyTOF
- Depletion
- FA
- FC
Description
| Product Name: | Anti-Mouse CD115 In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0001 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | AFS98 |
| Protein: | CD115 |
| Product Type: | Monoclonal Antibody |
| Synonyms: | Macrophage Colony Stimulating Factor Receptor, M-CSFR, CSF-R1, C-fms, CSF-1R |
| Isotype: | Rat IgG2a κ |
| Reactivity: | Mouse |
| Immunogen: | Not available |
| Applications: | B, CyTOF®, Depletion, FA, FC |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | B, CyTOF®, Depletion, FA, FC |
| Recommended Usage: | FC The suggested concentration for this AFS98 antibody for staining cells in flow cytometry is ≤ 0.25 µg per 106 in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application. |
| Reactivity: | Mouse |
| Host Species: | Rat |
| Specificity: | Clone AFS98 recognizes an epitope on mouse CD115. |
| Antigen Distribution: | CD115 is expressed on monocytes/macrophages, peritoneal exudate cells, plasmacytoid cells, dendritic cells, and osteoclasts. |
| Immunogen: | Not available |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
CD115 antibody, clone AFS98, recognizes CD115, also known as mouse colony-stimulating factor 1 receptor (CSF-1R and macrophage colony-stimulating factor receptor (M-CSFR). CD115 is a 150kDa single-pass type I membrane protein encoded by the c-fms gene that belongs to the type III protein tyrosine kinase receptor family. CD115 has an immunoglobulin-like extracellular domain, transmembrane domain, and C-terminal tail receptor and is expressed by monocytes, macrophages, plasmacytoid and conventional dendritic cells (DCs), osteoclasts, and their precursors. CD115 is the receptor for CSF1, also known as M-CSF, and IL-34. Binding and signaling through CD115 regulates the proliferation, differentiation, survival, and cytokine-production of monocytes and macrophages1,2. In addition, CD115 plays a key role in the differentiation and proliferation of osteoclasts as well as their bone resorption activity3. Mutations in CSF-1R are associated with cancer, including myeloid malignancies, Alzheimer’s disease, and other inflammatory and autoimmune diseases4. The presence of tumor-associated macrophages (TAMs) expressing CSF-1R correlates with poor survival in various tumor types5,6, and efforts to eliminate these TAMs using CSF1R small-molecule inhibitors and monoclonal antibodies are currently in clinical trials7.
| Technical Datasheet: | View |
| Protein: | CD115 |
| Ligand/Receptor: | Macrophage colony stimulating factor (M-CSF), IL-34 |
| Research Area: | Immunology |
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