Anti-MMP3 Antibody (CAB1202)
- SKU:
- CAB1202
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-MMP3 Antibody |
| Antibody SKU: | CAB1202 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 248-477 of human MMP3 (NP_002413.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 248-477 of human MMP3 (NP_002413.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | RFRL SQDD INGI QSLY GPPP DSPE TPLV PTEP VPPE PGTP ANCD PALS FDAV STLR GEIL IFKD RHFW RKSL RKLE PELH LISS FWPS LPSG VDAA YEVT SKDL VFIF KGNQ FWAI RGNE VRAG YPRG IHTL GFPP TVRK IDAA ISDK EKNK TYFF VEDK YWRF DEKR NSME PGFP KQIA EDFP GIDS KIDA VFEE FGFF YFFT GSSQ LEFD PNAK KVTH TLKS NSWL NC |
| Gene ID: | 4314 |
| Uniprot: | P08254 |
| Cellular Location: | Secreted, extracellular matrix, extracellular space |
| Calculated MW: | 53kDa |
| Observed MW: | 54kDa |
| Synonyms: | MMP3, CHDS6, MMP-3, SL-1, STMY, STMY1, STR1 |
| Background: | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans. The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. |
| UniProt Protein Function: | MMP3: Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase. Defects in MMP3 are the cause of susceptibility to coronary heart disease type 6 (CHDS6). A multifactorial disease characterized by an imbalance between myocardial functional requirements and the capacity of the coronary vessels to supply sufficient blood flow. Decreased capacity of the coronary vessels is often associated with thickening and loss of elasticity of the coronary arteries. A polymorphism in the MMP3 promoter region is associated with the risk of coronary heart disease and myocardial infarction, due to lower MMP3 proteolytic activity and higher extracellular matrix deposition in atherosclerotic lesions. Belongs to the peptidase M10A family. |
| UniProt Protein Details: | Protein type:Secreted; EC 3.4.24.17; Motility/polarity/chemotaxis; Secreted, signal peptide; Protease Chromosomal Location of Human Ortholog: 11q22.3 Cellular Component: proteinaceous extracellular matrix; extracellular space; mitochondrion; extracellular region; cytosol Molecular Function:zinc ion binding; metalloendopeptidase activity; endopeptidase activity; calcium ion binding Biological Process: extracellular matrix disassembly; positive regulation of protein oligomerization; collagen catabolic process; extracellular matrix organization and biogenesis; negative regulation of protein kinase B signaling cascade; protein catabolic process; proteolysis; regulation of cell migration Disease: Coronary Heart Disease, Susceptibility To, 6 |
| NCBI Summary: | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans. The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P08254 |
| NCBI GenInfo Identifier: | 116857 |
| NCBI Gene ID: | 4314 |
| NCBI Accession: | P08254.2 |
| UniProt Secondary Accession: | P08254,Q3B7S0, Q6GRF8, B2R8B8, |
| UniProt Related Accession: | P08254 |
| Molecular Weight: | 53,977 Da |
| NCBI Full Name: | Stromelysin-1 |
| NCBI Synonym Full Names: | matrix metallopeptidase 3 (stromelysin 1, progelatinase) |
| NCBI Official Symbol: | MMP3 |
| NCBI Official Synonym Symbols: | SL-1; STMY; STR1; CHDS6; MMP-3; STMY1 |
| NCBI Protein Information: | stromelysin-1; transin-1; proteoglycanase; matrix metalloproteinase-3; matrix metalloproteinase 3 (stromelysin 1, progelatinase) |
| UniProt Protein Name: | Stromelysin-1 |
| UniProt Synonym Protein Names: | Matrix metalloproteinase-3; MMP-3; Transin-1 |
| Protein Family: | Stromelysin |
| UniProt Gene Name: | MMP3 |
| UniProt Entry Name: | MMP3_HUMAN |
 | Western blot analysis of extracts of Mouse heart, using MMP3 antibody (CAB1202) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of C6 cells using MMP3 Polyclonal Antibody (CAB1202) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using MMP3 Polyclonal Antibody (CAB1202) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
Related Products
Anti-MYH10 Antibody (CAB12029)
