Anti-MMP20 Antibody (CAB9788)
- SKU:
- CAB9788
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-MMP20 Antibody |
| Antibody SKU: | CAB9788 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 108-310 of human MMP20 (NP_004762.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:1000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, mouse brain, rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 108-310 of human MMP20 (NP_004762.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | YRLF PGEP KWKK NTLT YRIS KYTP SMSS VEVD KAVE MALQ AWSS AVPL SFVR INSG EADI MISF ENGD HGDS YPFD GPRG TLAH AFAP GEGL GGDT HFDN AEKW TMGT NGFN LFTV AAHE FGHA LGLA HSTD PSAL MYPT YKYK NPYG FHLP KDDV KGIQ ALYG PRKV FLGK PTLP HAPH HKPS IPDL CDSS SSFD AVTM LGK |
| Gene ID: | 9313 |
| Uniprot: | O60882 |
| Cellular Location: | Secreted, extracellular matrix, extracellular space |
| Calculated MW: | 54kDa |
| Observed MW: | 54kDa |
| Synonyms: | MMP20, AI2A2, MMP-20 |
| Background: | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene degrades amelogenin, the major protein component of dental enamel matrix, and thus thought to play a role in tooth enamel formation. A mutation in this gene, which alters the normal splice pattern and results in premature termination of the encoded protein, has been associated with amelogenesis imperfecta. This gene is part of a cluster of MMP genes located on chromosome 11q22.3. |
| UniProt Protein Function: | MMP20: Degrades amelogenin, the major protein component of the enamel matrix and two of the macromolecules characterizing the cartilage extracellular matrix: aggrecan and the cartilage oligomeric matrix protein (COMP). May play a central role in tooth enamel formation. Defects in MMP20 are the cause of amelogenesis imperfecta hypomaturation type 2A2 (AI2A2). AI2A2 is an autosomal recessive defect of enamel formation. The disorder involves both primary and secondary dentitions. The teeth have a shiny agar jelly appearance and the enamel is softer than normal. Brown pigment is present in middle layers of enamel. Belongs to the peptidase M10A family. |
| UniProt Protein Details: | Protein type:EC 3.4.24.-; Secreted, signal peptide; Secreted; Protease Chromosomal Location of Human Ortholog: 11q22.3 Cellular Component: extracellular region; extracellular space Molecular Function:metalloendopeptidase activity; protein binding; serine-type endopeptidase activity Biological Process: collagen catabolic process Disease: Amelogenesis Imperfecta, Hypomaturation Type, Iia2 |
| NCBI Summary: | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene degrades amelogenin, the major protein component of dental enamel matrix, and thus thought to play a role in tooth enamel formation. A mutation in this gene, which alters the normal splice pattern and results in premature termination of the encoded protein, has been associated with amelogenesis imperfecta. This gene is part of a cluster of MMP genes located on chromosome 11q22.3. [provided by RefSeq, Aug 2011] |
| UniProt Code: | O60882 |
| NCBI GenInfo Identifier: | 322510116 |
| NCBI Gene ID: | 9313 |
| NCBI Accession: | O60882.3 |
| UniProt Secondary Accession: | O60882,Q6DKT9, |
| UniProt Related Accession: | O60882 |
| Molecular Weight: | 54,387 Da |
| NCBI Full Name: | Matrix metalloproteinase-20 |
| NCBI Synonym Full Names: | matrix metallopeptidase 20 |
| NCBI Official Symbol: | MMP20 |
| NCBI Official Synonym Symbols: | AI2A2; MMP-20 |
| NCBI Protein Information: | matrix metalloproteinase-20 |
| UniProt Protein Name: | Matrix metalloproteinase-20 |
| UniProt Synonym Protein Names: | Enamel metalloproteinase; Enamelysin |
| Protein Family: | Matrix metalloproteinase |
| UniProt Gene Name: | MMP20 |
| UniProt Entry Name: | MMP20_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MMP20 antibody (CAB9788) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
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