Anti-MMP16 항체 (CAB10409)

Antibody Name:	Anti-MMP16 Antibody
Antibody SKU:	CAB10409
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 290-565 of human MMP16 (NP_005932.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, HT-29, MCF7, HT-1080, Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 290-565 of human MMP16 (NP_005932.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	YGPP DKIP PPTR PLPT VPPH RSIP PADP RKND RPKP PRPP TGRP SYPG AKPN ICDG NFNT LAIL RREM FVFK DQWF WRVR NNRV MDGY PMQI TYFW RGLP PSID AVYE NSDG NFVF FKGN KYWV FKDT TLQP GYPH DLIT LGSG IPPH GIDS AIWW EDVG KTYF FKGD RYWR YSEE MKTM DPGY PKPI TVWK GIPE SPQG AFVH KENG FTYF YKGK EYWK FNNQ ILKV EPGY PRSI LKDF MGCD GPTD RVKE GHSP PDDV DIVI KLDN TAST VKAI
Gene ID:	4325
Uniprot:	P51512
Cellular Location:	Cell membrane, Cell surface, Extracellular side, Secreted, Single-pass type I membrane protein, extracellular matrix, extracellular space
Calculated MW:	52kDa/69kDa
Observed MW:	60kDa

Synonyms:

MMP16, C8orf57, MMP-X2, MT-MMP2, MT-MMP3, MT3-MMP

Background:

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The encoded protein activates MMP2 by cleavage. This gene was once referred to as MT-MMP2, but was renamed as MT-MMP3 or MMP16.

UniProt Protein Function:	MMP16: Endopeptidase that degrades various components of the extracellular matrix, such as collagen type III and fibronectin. Activates progelatinase A. Involved in the matrix remodeling of blood vessels. Isoform short cleaves fibronectin and also collagen type III, but at lower rate. It has no effect on type I, II, IV and V collagen. However, upon interaction with CSPG4, it may be involved in degradation and invasion of type I collagen by melanoma cells. Belongs to the peptidase M10A family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 3.4.24.-; Membrane protein, integral; Protease Chromosomal Location of Human Ortholog: 8q21.3 Cellular Component: Golgi lumen; integral to plasma membrane; plasma membrane Molecular Function:enzyme activator activity; metalloendopeptidase activity; zinc ion binding Biological Process: extracellular matrix disassembly; protein metabolic process; protein processing; proteolysis
NCBI Summary:	Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The encoded protein activates MMP2 by cleavage. This gene was once referred to as MT-MMP2, but was renamed as MT-MMP3 or MMP16. [provided by RefSeq, Oct 2010]
UniProt Code:	P51512
NCBI GenInfo Identifier:	3041669
NCBI Gene ID:	4325
NCBI Accession:	P51512.2
UniProt Secondary Accession:	P51512,Q14824, Q52H48, B2RAN7,
UniProt Related Accession:	P51512
Molecular Weight:	Observed MW: 60kDaCalculated MW: 52kDa/69kDa
NCBI Full Name:	Matrix metalloproteinase-16
NCBI Synonym Full Names:	matrix metallopeptidase 16
NCBI Official Symbol:	MMP16
NCBI Official Synonym Symbols:	MMP-X2; C8orf57; MT-MMP2; MT-MMP3; MT3-MMP
NCBI Protein Information:	matrix metalloproteinase-16
UniProt Protein Name:	Matrix metalloproteinase-16
UniProt Synonym Protein Names:	MMP-X2; Membrane-type matrix metalloproteinase 3; MT-MMP 3; MTMMP3; Membrane-type-3 matrix metalloproteinase; MT3-MMP; MT3MMP
Protein Family:	Matrix metalloproteinase
UniProt Gene Name:	MMP16

	Western blot analysis of extracts of various cell lines, using MMP16 antibody (CAB10409) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded human lung cancer using MMP16 antibody (CAB10409) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon carcinoma using MMP16 antibody (CAB10409) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human breast using MMP16 antibody (CAB10409) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human breast cancer using MMP16 antibody (CAB10409) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
MMP16 Antibody (PACO01118)	Human MMP-16 ELISA Kit
	Human Matrix metalloproteinase-16 (MMP16) ELISA Kit
	MMP-16 Colorimetric Cell-Based ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)